Phospho-p53 (Ser376) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1699
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Phospho-p53
(Ser376) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB
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H
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53.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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-
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-
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-
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-
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Phospho-p53
(Ser376) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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7157(Human)
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SwissProt
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P04637(Human)
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Synonyms
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BCC7; LFS1;
P53; TRP53
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Category
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Cell Cycle
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Background
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p53, a
DNA-binding, oligomerization domain- and transcription activation
domain-containing tumor suppressor, upregulates growth arrest and
apoptosis-related genes in response to stress signals, thereby influencing
programmed cell death, cell differentiation, and cell cycle control
mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the
cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase
that is upregulated in the presence of active p53, where it
poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between
latent and active DNA-binding conformations and is differentially activated
through posttranslational modifications, including phosphorylation and
acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids
110-286, can compromise energetically-favorable association with cis elements
and are implicated in several human cancers.
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包装清单:
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产品编号
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产品名称
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包装
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XY1699
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Phospho-p53
(Ser376) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Phospho-p53
(Ser376) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10