Caldesmon Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1579
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Caldesmon
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, R
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70.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Caldesmon
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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800(Human);
109624(Mouse); 25687(Rat)
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SwissProt
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Q05682(Human);
Q62736(Rat)
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Synonyms
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CDM; H-CAD;
HCAD; L-CAD; LCAD; NAG22
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Category
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Cytoskeletal
Signaling
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Background
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Caldesmon,
Filamin 1, Nebulin and Villin are differentially expressed and regulated
Actin binding proteins. Both muscular and non-muscular forms of Caldesmon
have been identified and each has been shown to bind to Actin as well as to
calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon
results in five isoforms. Muscular Caldesmon (isoform 1), also designated
high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed
predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon
(isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon
(L-CAD), is widely expressed in non-muscle tissues and cells. Filamin 1,
which is ubiquitously expressed and exists as a homodimer, functions to
crosslink Actin to filaments. Nebulin is a large filamentous protein specific
to muscle tissue that may function as a ruler for filament length. Several
isoforms of Nebulin are produced by alternative exon usage. Villin is
Ca2+-regulated and is the major structural component of the brush border of
absorptive cells.
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包装清单:
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产品编号
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产品名称
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包装
|
|
XY1579
|
Caldesmon
Rabbit Monoclonal Antibody
|
50μl
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XY050
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Western一抗稀释液
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50ml
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|
-
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说明书
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1份
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保存条件:
Caldesmon Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10