Topoisomerase II alpha Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1525
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Topoisomerase
II alpha Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IHC
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H, M, R
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174.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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-
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1:50-1:200
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-
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Topoisomerase
II alpha Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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7153(Human);
21973(Mouse); 360243(Rat)
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SwissProt
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P11388(Human);
Q01320(Mouse); P41516(Rat)
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Synonyms
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DNA
topoisomerase 2-alpha; TOP2A; TOP2
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Category
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Cancer Biology
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Background
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DNA
topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate
the topological structure of DNA in eukaryotic cells by transiently breaking
and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing
both positive and negative supercoils, whereas prokaryotic topoisomerases
relax only negative supercoils. DNA topoisomerases play a role in DNA
replication, recombination, and transcription and have been identified as
targets of numerous anticancer drugs. Topo I, a ubiquitously expressed,
soluble enzyme, acts by introducing a transient break in one strand of DNA,
while Topo II acts by making a transient double-strand break. Topo II is
encoded by two different genes to generate two distinct isoforms that are
designated Topo IIα and Topo IIβ. Topo IIβ and Topo IIα, are largely homologous at
their N-terminal three quarters, however, the C-terminal segments are
considerably divergent, suggesting that these regions may mediate different
cellular functions and account for the observed differential tissue
expression patterns of the two isoforms.
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包装清单:
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产品编号
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产品名称
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包装
|
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XY1525
|
Topoisomerase
II alpha Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Topoisomerase II
alpha Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10