Phospho-p95/NBS1 (Ser343) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1513
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Phospho-p95/NBS1
(Ser343) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H
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95.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Phospho-p95/NBS1
(Ser343) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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4683(Human)
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SwissProt
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O60934(Human)
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Synonyms
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AT-V1; AT-V2;
ATV; NBS; NBS1; P95
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Category
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Cell Cycle
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Background
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Nijmegen
breakage syndrome (NBS) is characterized by extreme radiation sensitivity,
chromosomal instability and cancer. These phenotypes are similar to those of
ataxia telangiectasia mutated (ATM) disease, where there is a deficiency in a
protein kinase that is activated by DNA damage, indicating that the NBS1
(Nibrin) and ATM proteins may participate in common pathways. Nibrin is
specifically phosphorylated in response to gamma-radiation, ultraviolet light
and exposure to hydroxyurea. The phosphorylation of Nibrin requires
catalytically active ATM. ATM physically interacts with and phosphorylates
Nibrin on Serine 343 both in vitro and in vivo. Serine 343 is phosphorylated
in vitro by ATM and the modification of this residue in vivo is essential for
the cellular response to DNA damage. This response includes S-phase
checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and
rescue of hypersensitivity to ionizing radiation.
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包装清单:
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产品编号
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产品名称
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包装
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XY1513
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Phospho-p95/NBS1
(Ser343) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Phospho-p95/NBS1
(Ser343) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10