NADPH oxidase 4/NOX4 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1498
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NADPH oxidase
4/NOX4 Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H, M, R
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67.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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1:10-1:50
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1:50-1:100
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1:50-1:200
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1:50-1:100
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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NADPH oxidase
4/NOX4 Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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50507(Human);
85431(Rat)
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SwissProt
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Q9NPH5(Human);
Q924V1(Rat)
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Synonyms
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KOX; KOX-1;
RENOX
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Category
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Metabolism
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Background
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The
superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome
containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins
p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and
p67-phox migrate to the plasma membrane where they associate with the
flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22
and gp91-phox subunits also function as surface O2 sensors that initiate
cellular signaling in response to hypoxic conditions. Nox4 (also known as
Renox) is a renal gp91-phox homolog highly expressed at the site of
erythropoietin production in the proximal convoluted tubule epithelial cells
of the renal cortex. Nox4 is also expressed in fetal tissues, placenta,
glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of
Nox4 produces superoxide anions. In vascular cells, the addition of
Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in
vascular oxidative stress response. The gene encoding human Nox4 maps to
chromosome 11q14.2-q21.
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包装清单:
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产品编号
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产品名称
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包装
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XY1498
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NADPH oxidase
4/NOX4 Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
NADPH oxidase
4/NOX4 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10