CD3 epsilon Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1480
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CD3 epsilon
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IHC,
FC
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H
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23.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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-
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1:50-1:200
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-
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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CD3 epsilon
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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916(Human)
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SwissProt
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P07766(Human)
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Synonyms
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IMD18; T3E;
TCRE
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Category
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Immunology/Inflamation
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Background
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The T cell
antigen receptor (TCR) recognizes foreign antigens and translates such
recognition events into intracellular signals that elicit a change in the
cell from a dormant to an activated state. Much of this signaling process can
be attributed to a multisubunit complex of proteins that associates directly
with the TCR. This complex has been designated CD3 (cluster of
differentiation 3). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor
tyrosine-based activation motif (ITAM). Engagement of TCR complex with
foreign antigens induces tyrosine phosphorylation in the ITAM motifs and
phosphorylated ITAMs function as docking sites for signaling molecules such
as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a
conformational change in CD3ε, such that a proline
region is exposed and then associates with the adaptor protein Nck.
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包装清单:
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产品编号
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产品名称
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包装
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XY1480
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CD3 epsilon
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
CD3 epsilon
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10