Phospho-PKR (Thr446) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1474
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Phospho-PKR
(Thr446) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IHC,
ChIP
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H
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62.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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-
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1:50-1:200
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-
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-
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-
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1:10-1:50
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抗体详细信息如下:
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About this
Antibody
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Name
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Phospho-PKR
(Thr446) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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5610(Human)
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SwissProt
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P19525(Human)
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Synonyms
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EIF2AK1; PKR;
PPP1R83; PRKR
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Category
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Others
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Background
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An
interferon-inducible, RNA-dependent protein serine/threonine kinase, PKR has
various designations. Mouse PKR is known as DAI, dsJ, PI kinase, p65, p67 or
TIK, whereas human PKR is known as p68 or p69. PKR phosphorylates its
substrate, a subunit of protein synthesis initiation factor eIF-2 on Ser 51
to inhibit translation. PKR contains two dsRNA binding motifs required for
its activation by dsRNA. Three kinds of regulation of PKR enzymatic activity
occur, and these include transcriptional regulation in response to
interferon, an autoregulatory mechanism controlling PKR expression at the
level of translation, and posttranslational regulation by RNA mediated
autophosphorylation. Human PKR contains at least 15 autophosphorylation
sites, but only Thr-446 and Thr-451 in the activation loop are critical for
its kinase activity. Thr-446 is the in vivo autophosphorylation site of PKR.
Mutation of threonine to alanine at position 446 substantially reduces PKR
function, and mutant kinase containing Ala-451 is completely inactive.
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包装清单:
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产品编号
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产品名称
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包装
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XY1474
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Phospho-PKR
(Thr446) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Phospho-PKR
(Thr446) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10