PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1336
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PHD1/prolyl
hydroxylase Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC, FC
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H, M, R
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44.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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PHD1/prolyl
hydroxylase Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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112398(Human);
112406(Mouse); 308457(Rat)
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SwissProt
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Q96KS0(Human);
Q91YE2(Mouse); Q6AYU4(Rat)
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Synonyms
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Category
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Angiogenesis;
Epigenetics
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Background
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Prolyl
hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1,
PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-α subunits. Hypoxia-inducible factor (HIF) is a transcriptional
regulator important in several aspects of oxygen homeostasis. The prolyl
hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in
HIF-α proteins. HIF PHD1, which is widely expressed,
with highest levels of expression in testis, functions as a cellular oxygen
sensor and is important in cell growth regulation. HIF PHD1 can localize to
the nucleus or the cytoplasm and is also detected in hormone responsive
tissues, such as normal and cancerous mammary, ovarian and prostate
epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3.
HIF PHD2 is regarded as the main cellular oxygen sensor, as RNA interference
against HIF PHD2, but not HIF PHD1 or HIF PHD3, is enough to stabilize HIF-1α in normoxia. HIF PHD2, a direct HIF target gene, is expressed
mainly in skeletal muscle, heart, kidney and brain. HIF PHD3 may play a role
in the regulation of cell growth in muscle cells and in apoptosis in neuronal
tissue. HIF PHD3 is widely expressed, although the highest levels can be
detected in placenta and heart.
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包装清单:
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产品编号
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产品名称
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包装
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XY1336
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PHD1/prolyl
hydroxylase Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
PHD1/prolyl
hydroxylase Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10