Cytokeratin 18 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1285
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Cytokeratin 18
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC, FC
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H, M, R
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48.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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-
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1:10-1:50
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1:5-1:20
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1:10-1:50
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1:5-1:10
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Cytokeratin 18
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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3875(Human);
16668(Mouse); 294853(Rat)
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SwissProt
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P05783(Human);
P05784(Mouse); Q5BJY9(Rat)
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Synonyms
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K18; CK-18;
CYK18
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Category
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Cytoskeletal
Signaling
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Background
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Cytokeratins
comprise a diverse group of intermediate filament proteins (IFPs) that are
expressed as pairs in both keratinized and non-keratinized epithelial tissue.
Cytokeratins play a critical role in differentiation and tissue
specialization and function to maintain the overall structural integrity of
epithelial cells. Cytokeratins have been found to be useful markers of tissue
differentiation which is directly applicable to the characterization of
malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in
a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a
majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18
contains two major phosphorylation sites on Ser 33 and Ser 52.
Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18
with 14-3-3 proteins and is involved in keratin organization and
distribution.
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包装清单:
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产品编号
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产品名称
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包装
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XY1285
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Cytokeratin 18
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Cytokeratin 18
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10