SIRT1 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1267
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SIRT1 Rabbit
Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H
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110.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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SIRT1 Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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23411(Human)
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SwissProt
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Q96EB6(Human)
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Synonyms
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SIR2L1
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Category
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Epigenetics;
DNA Damage
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Background
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The silent
information regulator (SIR2) family of genes are highly-conserved from
prokaryotes to eukaryotes and are involved in diverse processes, including
transcriptional regulation, cell cycle progression, DNA-damage repair and
aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent
manner, which regulates silencing at the telomeric, rDNA and silent
mating-type loci. Sir2p is the founding member of a large family, designated
sirtuins, which contain a conserved catalytic domain. The human homologs,
which include SIRT1-7, are divided into four main branches: SIRT1-3 are class
I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has
the closest homology to the yeast Sir2p and is widely expressed in fetal and
adul tissues. SIRT1 is highly expressed in heart, brain and skeletal muscle,
with low expression in lung and placenta. SIRT1 regulates the p53-dependent
DNA damage response pathway by binding to and deacetylating p53, specifically
at Lys 382.
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包装清单:
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产品编号
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产品名称
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包装
|
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XY1267
|
SIRT1 Rabbit
Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
SIRT1 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10