Caspase-8 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1243
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Caspase-8
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC
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H
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55.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Caspase-8
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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841(Human)
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SwissProt
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Q14790(Human)
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Synonyms
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ALPS2B; CAP4;
Casp-8; FLICE; MACH; MCH5
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Category
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Apoptosis
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Background
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Initiator
caspases, which include caspase-8, activate effector caspases by cleaving
inactive forms of effector caspases. In the activation cascade responsible
for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is
the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming
an aggregate referred to as death-inducing signaling complex (DISC), which
activates caspase-8. The actived protein is released from the complex and
further activates downstream apoptotic proteases. Caspase-8, which is a
heterodimer consisting of two subunits (p18 and p10), is widely expressed,
but is detected at highest levels in peripheral blood leukocytes (PBLs),
thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8,
may cause CASP8D (caspase-8 deficiency disorder), which is characterized by
splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency
due to defects in T lymphocyte, NK cell and B lymphocyte activation.
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包装清单:
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产品编号
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产品名称
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包装
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XY1243
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Caspase-8
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Caspase-8 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10