Phospho-eIF-2a (Ser51) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1237
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Phospho-eIF-2a
(Ser51) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, R
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36.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Phospho-eIF-2a
(Ser51) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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83939(Human);
229317(Mouse); 502531(Rat)
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SwissProt
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Q9BY44(Human);
Q8BJW6(Mouse)
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Synonyms
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EIF-2; EIF-2A;
EIF-2alpha; EIF2; EIF2A
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Category
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Others
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Background
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Phosphorylation
of the eukaryotic initiation factor 2 (eIF2) α subunit is a
well-documented mechanism to downregulate protein synthesis under a variety
of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi
and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation
complex. eIF2 promotes a new round of translation initiation by exchanging
GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by
viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid
deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the
eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR
by IFN-γ and TNF-α induces
potent phosphorylation of eIF2α at Ser51.
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包装清单:
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产品编号
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产品名称
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包装
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XY1237
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Phospho-eIF-2a
(Ser51) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Phospho-eIF-2a
(Ser51) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10