NF-kB p65 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1234
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NF-kB p65
Rabbit Monoclonal Antibody
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50μl
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617.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, Z
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65.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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NF-kB p65
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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5970(Human);
19697(Mouse)
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SwissProt
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Q04206(Human);
Q04207(Mouse)
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Synonyms
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NFKB3; p65
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Category
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Immunology/Inflamation
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Background
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Proteins
encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are
members of a family of transcription factors that include the two subunits of
the transcription factor NF kB (p50 and p65) and the Drosophila maternal
morphogen, dorsal. Both proteins specifically bind to DNA sequences that are
the same or slight variations of the 10 bp kB sequence in the immunoglobulin
k light chain enhancer. This same sequence is also present in a number of
other cellular and viral enhancers. The DNA binding activity of NFkB is
activated and NFkB is subsequently transported from the cytoplasm to the
nucleus in cells exposed to mitogens or growth factors. cDNAs encoding
precursors for two distinct proteins of the same size have been described,
designated p105 and p100. The p105 precursor contains p50 at its N-terminus
and a C-terminal region that when expressed as a separate molecule,
designated pdI, binds to p50 and regulates its activity.
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包装清单:
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产品编号
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产品名称
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包装
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XY1234
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NF-kB p65
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
NF-kB p65 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10