Phospho-Histone H2A.X (Ser139) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1201
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Phospho-Histone
H2A.X (Ser139) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IHC
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H, M, R
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15.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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-
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-
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1:50-1:200
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-
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Phospho-Histone
H2A.X (Ser139) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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3014(Human);
15270(Mouse)
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SwissProt
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P16104(Human);
P27661(Mouse)
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Synonyms
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H2A.X; H2A/X;
H2AX
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Category
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Epigenetics
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Background
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Histone H2A.X
is a member of the Histone H2A family, which is involved in nucleosomal
organization of chromatin. The H2XYX gene is located in close proximity to
the Porphobilinogen deaminase (PBG-D) gene in both mouse and human, and maps
to chromosome 9 and 11q23, respectively. H2A.X differs from the other members
of the H2A family by the presence of a highly conserved C-terminal motif. It
is rapidly phosphorylated in response to ionizing radiation and plays an
important role in the recognition and repair of DNA double stranded breaks.
The phosphorylated form of H2A.X, designated γ-H2A.X, forms
nuclear foci at the heavy chain constant region of cells involved in class
switch recombination (CSR), a region-specific DNA reaction that replaces one
immunoglobulin heavy chain constant region gene with another. The
phosphorylated γ-H2A.X is also thought to initiate
subsequent repair factors, including Rad50, Rad51 and BRCA1.
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包装清单:
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产品编号
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产品名称
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包装
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XY1201
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Phospho-Histone
H2A.X (Ser139) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Phospho-Histone
H2A.X (Ser139) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10