Mono-Methyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1165
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Mono-Methyl-Histone
H3 (Lys18) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC
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H, M, R
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15.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Mono-Methyl-Histone
H3 (Lys18) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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8350/8351/8352/8353/8354/8355/8356/8357/8358/8968/3020/3021/8290/440093/126961/
333932/653604(Human); 319152/319153/360198/97908(Mouse);
291159/679994/680498(Rat)
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SwissProt
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P68431/P84243/Q16695/Q6NXT2/Q71DI3(Human);
P68433(Mouse); Q6LED0(Rat)
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Synonyms
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H3.4; H3/g;
H3FT; H3t
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Category
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Epigenetics
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Background
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Eukaryotic
histones are basic and water soluble nuclear proteins that form
hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a
left-handed super-helical turn sequentially to form chromosomal fibers. Two
molecules of each of the four core histones (H2A, H2B, H3 and H4) form the
octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers,
forming two nearly symmetrical halves by tertiary structure. Histones are
subject to posttranslational modification by enzymes primarily on their
N-terminal tails, but also in their globular domains. Human and mouse Histone
H4 are subject to methylation at Lys 20, a modification that may be necessary
for select DNA transactions or chromatin state transitions.
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包装清单:
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产品编号
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产品名称
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包装
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XY1165
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Mono-Methyl-Histone
H3 (Lys18) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Mono-Methyl-Histone
H3 (Lys18) Rabbit Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验,请注意回收使用过的稀释抗体?;厥盏目固逋ǔV辽倏梢灾馗词褂?span>5-10