Mono-Methyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1153
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Mono-Methyl-Histone
H3 (Lys36) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
ICC
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H, M
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15.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000-1:2000
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Mono-Methyl-Histone
H3 (Lys36) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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8350/3020/8290/440093/126961(Human);
15078(Mouse)
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SwissProt
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P68431/P84243/Q16695/Q6NXT2/Q71DI3(Human);
P84244(Mouse)
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Synonyms
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H3/A; H3FA
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Category
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Epigenetics
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Background
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In eukaryotes,
DNA is wrapped around histone octamers to form the basic unit of chromatin
structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it
associates with approximately 200 base pairs of DNA to form the nucleosome.
The association of DNA with histones results in dense packing of chromatin,
which restricts proteins involved in gene transcription from binding to DNA.
Histone H3, the core protein of the nucleosome, becomes phosphorylated at the
end of prophase. The two major sites of phosphorylation are the
mitosis-specific site Ser10, and Ser28, both of which are extensively
phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone
H3. The nucleosome structure of histone H3 promotes N-terminal
phosphorylation in vitro.
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包装清单:
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产品编号
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产品名称
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包装
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XY1153
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Mono-Methyl-Histone
H3 (Lys36) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Mono-Methyl-Histone
H3 (Lys36) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10