Phospho-Rb (Ser807) Rabbit Monoclonal Antibody
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY1135
|
Phospho-Rb
(Ser807) Rabbit Monoclonal Antibody
|
50μl
|
618.00元
|
|
来源
|
用途
|
交叉反应性
|
分子量
|
|
Rabbit
|
WB, IP, IF,
IHC, ICC
|
H, M, R
|
106.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1000-1:2000
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
-
|
-
|
-
|
抗体详细信息如下:
|
About this
Antibody
|
|
Name
|
Phospho-Rb
(Ser807) Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
5925(Human);
19645(Mouse); 24708(Rat)
|
|
SwissProt
|
P06400(Human);
P13405(Mouse); P33568(Rat)
|
|
Synonyms
|
RB; pRb; OSRC;
pp110; p105-Rb; PPP1R130
|
|
Category
|
MAPK
|
|
Background
|
Pediatric
cancer retinoblastoma and the formation of other human tumors can be
attributed to mutations in the retinoblastoma tumor suppressor gene (Rb). The
Rb protein regulates differentiation, apoptosis and cell cycle control by
coordinating the cell cycle at G1-S with transcriptional machinery. During
G1, cyclin D-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks
the conversion of Rb from a transcriptionally repressive, hypophosphorylated
state to an inactive, phosphorylated state, which may be sustained through
mitosis by differential phosphorylation of up to 16 putative serine or
threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780,
Ser-807/ Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the
transcription of genes controlling the cell cycle through direct
protein-protein interactions and through the recruitment of histone
deacetylase.
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY1135
|
Phospho-Rb
(Ser807) Rabbit Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀释液
|
50ml
|
|
-
|
说明书
|
1份
|
保存条件:
Phospho-Rb
(Ser807) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10