Hsc70 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1132
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Hsc70 Rabbit
Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H, M, R
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70.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000-1:2000
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Hsc70 Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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3312(Human);
15481(Mouse); 24468(Rat)
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SwissProt
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P11142(Human);
P63017(Mouse); P63018(Rat)
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Synonyms
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HEL-33;
HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71
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Category
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Protein
Folding and TrXYficking
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Background
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The HSP 70
family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75
and GRP 78. These proteins serve a variety of roles: they act as molecular
chaperones facilitating the assembly of multi-protein complexes, participate
in the translocation of polypeptides across cell membranes and to the
nucleus, and aid in the proper folding of nascent polypeptide chains. All
members of the family, except HSP 70, are constitutively expressed in primate
cells. HSP 70 expression is strongly induced in response to heat stress. HSP
70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and
mitochondrial import machinery, and are found in both the cytosol and nucleus
of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of
nascent polypeptide chains and in protecting cells against the accumulation
of improperly folded proteins. GRP 78 is localized in the endoplasmic
reticulum, where it receives imported secretory proteins and is involved in
the folding and translocation of nascent peptide chains. GRP 75 expression is
restricted to the mitochondrial matrix and aids in the translocation and
folding of nascent polypeptide chains of both nuclear and mitochondrial
origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by
glucose deprivation. It has been postulated that members of the HSP 70 family
act as force-generating motors, relying on the hydrolysis of ATP for their
activity.
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包装清单:
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产品编号
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产品名称
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包装
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XY1132
|
Hsc70 Rabbit
Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Hsc70 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10