Prohibitin Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1126
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Prohibitin
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, R
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30.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000-1:2000
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1:50-1:200
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1:50-1:200
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1:200-1:500
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Prohibitin
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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5245(Human);
18673(Mouse); 25344(Rat)
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SwissProt
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P35232(Human);
P67778(Mouse); P67779(Rat)
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Synonyms
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PHB1; HEL-215;
HEL-S-54e
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Category
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Cell Cycle;
Apoptosis; Metabolism
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Background
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Prohibitin is
an evolutionarily conserved protein that has antiproliferative activity. The
gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously
expressed. Prohibitin is a post-synthetically modified protein that is localized
in the inner membrane of mitochondria, where it regulates the cell cycle by
blocking the transition between the G1 and S phases, and on the plasma
membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and
protein levels are high in G1, decline during the S phase, rise again in G2
and decline in M phase, which suggests that prohibitin controls the cell
cycle by using both transcriptional and posttranslational mechanisms.
Prohibitin is also a potential tumor suppressor protein that binds to
retinoblastoma (Rb) and subsequently inhibits the activity of E2F family
members in response to specific signaling cascades. Prohibitin 2 is a
repressor of estrogen receptor activity, and is required for somatic and
germline differentiation in the larval gonad during embryonic development.
Mutations in the Prohibitin genes are correlated with breast cancer
development and/or progressionin more than 80% of the cell lines analyzed.
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包装清单:
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产品编号
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产品名称
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包装
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XY1126
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Prohibitin
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Prohibitin
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10