Caspase-2 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1123
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Caspase-2
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, R
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48/30/12 kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000-1:2000
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Caspase-2
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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835(Human);
12366(Mouse); 64314(Rat)
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SwissProt
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P42575(Human);
P29594(Mouse); P55215(Rat)
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Synonyms
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ICH1; NEDD2;
CASP-2; NEDD-2; PPP1R57
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Category
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Apoptosis
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Background
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Caspase-2
(Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated
in response to various apoptotic stimuli. Caspase-2 is unique among the
caspases in that it has features of both upstream caspases (long prodomain)
and downstream caspases (DEXD substrate specificity). Caspase-2 is highly
expressed in the brain during development, and is expressed at low levels in
adul tissue. Specifically, caspase-2 localizes to the mitochondria, the
Golgi, the cytoplasm, and the nucleus. Caspase-2 exists as two isoforms,
caspase-2L and caspase-2S, which are produced by alternative splicing and
differ in their N and C-termini. Caspase-2L acts as a positive regulator of
apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis.
Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at
Asp-153 to produce a fragment (p30). The p30 fragment undergoes further
cleavage to generate a fragment containing amino acids 153-308 (p18) and a
fragment containing amino acids 317-435 (p13 or p14). As apoptosis
progresses, the p13 (p14) fragment can undergo further processing to yield a
fragment containing amino acids 331-435 (p12).
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包装清单:
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产品编号
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产品名称
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包装
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XY1123
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Caspase-2
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Caspase-2 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10