Vimentin Mouse Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0318
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Vimentin Mouse
Monoclonal Antibody
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50μl
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416.00元
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来源
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用途
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交叉反应性
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分子量
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Mouse
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WB, ICC, IHC,
FC, IF
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H, M, R
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54KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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1:100
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1:100
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1:100
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1:25-1:50
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Vimentin Mouse
Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgG1
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with a synthetic peptide (KLH-coupled)
corresponding to a region of Vimentin.
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Gene ID
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7431
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SwissProt
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P08670
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Synonyms
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VIM
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Category
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Cytoskeletal
Signaling
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Background
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Vimentin plays
a significant role in supporting and anchoring the position of the organelles
in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum,
and mitochondria, either laterally or terminally. In essence, vimentin is
responsible for maintaining cell shape, integrity of the cytoplasm, and
stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate
toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of
asymmetric division of mammalian cell lines. It has been used as a sarcoma
tumor marker to identify mesenchyme. Methylation of the vimentin gene has
been established as a biomarker of colon cancer and this is being utilized in
the development of fecal tests for colon cancer. High levels of DNA methylation
in the promotor region have also been associated with markedly decreased
survival in hormone positive breast cancers.
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包装清单：
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产品编号
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产品名称
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包装
|
|
XY0318
|
Vimentin Mouse
Monoclonal Antibody
|
50μl
|
|
AZ050
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Western一抗稀释液
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50ml
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|
—
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说明书
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1份
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保存条件：
Vimentin Mouse
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10