Ubiquitin Rabbit Polyclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0306
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Ubiquitin
Rabbit Polyclonal Antibody
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50μl
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416.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, ICC, IHC,
IF
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H, M, R
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10KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Ubiquitin
Rabbit Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to Ubiquitin.
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Gene ID
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7314
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SwissProt
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P62988
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Synonyms
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40S ribosomal
protein S27a; CEP80; Ribosomal protein S27a; RPS27A; UBA52; UBA80; UBB; UBC;
UBCEP1; UBCEP80; UBIQ; ubiquitin and ribosomal protein S27a; ubiquitin
carboxyl extension protein 80; ubiquitin-CEP80
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Category
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Ubiquitin and
Ubiquitin-like proteins
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Background
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Ubiquitin is a
conserved polypeptide unit that plays an important role in the
ubiquitin-proteasome pathway. Ubiquitin exists either covalently attached to
another protein, or free (unanchored). When covalently bound, it is
conjugated to target proteins via an isopeptide bond either as a monomer
(monoubiquitin), a polymer linked via different Lys residues of the ubiquitin
(polyubiquitin chains) or a linear polymer linked via the initiator Met of
the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when
attached to a target protein, have different functions depending on the Lys
residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA
repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated
degradation) and in cell-cycle regulation; Lys-29-linked is involved in
lysosomal degradation; Lys-33-linked is involved in kinase modification;
Lys-48-linked is involved in protein degradation via the proteasome;
Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in
signaling processes leading to activation of the transcription factor
NF-kappa-B.
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包装清单:
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产品编号
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产品名称
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包装
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XY0306
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Ubiquitin
Rabbit Polyclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件:
Ubiquitin Rabbit
Polyclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验,请注意回收使用过的稀释抗体?;厥盏目固逋ǔV辽倏梢灾馗词褂?span>5-10