NF-κB p65 Rabbit Polyclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0246
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NF-κB p65 Rabbit Polyclonal Antibody
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50μl
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412.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, FC, IHC
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H, M, R
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65KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000-1:2000
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-
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-
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1:100-1:200
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-
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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NF-κB p65 Rabbit Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to N-terminal NF-kB p65.
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Gene ID
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5970
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SwissProt
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Q04206
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Synonyms
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NFKB3; Nuclear
factor NF-kappa-B p65 subunit; RELA; TF65; Transcription factor p65; p65
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Category
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Immunology and
Inflammation
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Background
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NF-kappa-B is
a pleiotropic transcription factor present in almost all cell types and is
the endpoint of a series of signal transduction events that are initiated by
a vast array of stimuli related to many biological processes such as
inflammation, immunity, differentiation, cell growth, tumorigenesis and
apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the
Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50,
REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most
abundant one. In unstimulated cells, NF-κB is sequestered in
the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the
ubiquitin-proteasome pathway and releasing NF-κB to
enter the nucleus where it regulates gene expression .
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包装清单：
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产品编号
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产品名称
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包装
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XY0246
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NF-κB p65 Rabbit Polyclonal Antibody
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50μl
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AZ050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件：
NF-κB
p65 Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10