HMGB1 Rabbit Polyclonal Antibody
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY0180
|
HMGB1 Rabbit
Polyclonal Antibody
|
50μl
|
412.00元
|
|
来源
|
用途
|
交叉反应性
|
分子量
|
|
Rabbit
|
WB, FC, IHC,
ICC, IF
|
H, M, R
|
25KDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:500-1:1000
|
-
|
1:200
|
1:200
|
1:200
|
1:50-1:100
|
-
|
-
|
抗体详细信息如下:
|
About this
Antibody
|
|
Name
|
HMGB1 Rabbit
Polyclonal Antibody
|
|
Category
|
Polyclonal
antibody(pAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
Peptide XYfinity
purified
|
|
About the
Immunogen
|
|
Immunogen
|
This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to HMGB1.
|
|
Gene ID
|
3146
|
|
SwissProt
|
P09429
|
|
Synonyms
|
DKFZp686A04236;
HMG1; HMG3; SBP-1
|
|
Category
|
Chromatin
Regulation / Nuclear Function
|
|
Background
|
Like the
histones, HMGB1, also known as high-mobility group protein 1 (HMG-1) is among
the most important chromatin proteins. In the nucleus HMGB1 interacts with
nucleosomes, transcription factors, and histones. This nuclear protein
organizes the DNA and regulates transcription. XYter binding, HMGB1 bends
DNA, which facilitates the binding of other proteins. HMGB1 is secreted by
immune cells through leaderless secretory pathway. Activated macrophages and
monocytes secrete HMGB1 as a cytokine mediator of Inflammation. In recent
research, HMGB1 has been reported as a novel biomarker for human ovarian
cancer.
|
包装清单：
|
产品编号
|
产品名称
|
包装
|
|
XY0180
|
HMGB1 Rabbit
Polyclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀释液
|
50ml
|
|
—
|
说明书
|
1份
|
保存条件：
HMGB1 Rabbit
Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western 一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体 ?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10