E-Cadherin Mouse Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0138
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E-Cadherin
Mouse Monoclonal Antibody
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50μl
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416.00元
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来源
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用途
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交叉反应性
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分子量
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Mouse
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WB, ICC, IHC,
FC, IF
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H, M
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130KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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E-Cadherin
Mouse Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgG1
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with recombinant protein to a region of
E-cadherin.
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Gene ID
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999
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SwissProt
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P12830
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Synonyms
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CADH1;
cadherin 1; type 1; E-cadherin (epithelial) (ECAD); cadherin-1; CAM 120/80;
E-cadherin; epithelial-cadherin; uvomorulin
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Category
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Adhesion/ECM
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Background
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E-cadherin
(epithelial) is the most well-studied member of the cadherin family. It
consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one
transmembrane domain, and an intracellular domain that binds p120-catenin and
beta-catenin. The intracellular domain contains a highly-phosphorylated
region vital to beta-catenin binding and, therefore, to E-cadherin function.
Loss of E-cadherin function or expression has been implicated in cancer
progression and metastasis. E-cadherin downregulation decreases the strength
of cellular adhesion within a tissue, resulting in an increase in cellular
motility. This in turn may allow cancer cells to cross the basement membrane
and invade surrounding tissues. E-cadherin is also used by pathologists to
diagnose different kinds of breast cancer.
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包装清单：
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产品编号
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产品名称
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包装
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XY0138
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E-Cadherin
Mouse Monoclonal Antibody
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50μl
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AZ050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件：
E-Cadherin Mouse
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10