CRISPR-Cas9 Mouse Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0123
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CRISPR-Cas9
Mouse Monoclonal Antibody
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50μl
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416.00元
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来源
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用途
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交叉反应性
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分子量
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Mouse
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WB, ICC, IF
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-
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-
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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1:40
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-
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1:40
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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CRISPR-Cas9
Mouse Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgM
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with a recombinant protein corresponding to a
sequence of CRISPR-Cas9.
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Gene ID
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-
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SwissProt
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-
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Synonyms
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-
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Category
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-
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Background
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The Clustered
Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is
currently the most commonly used RNA-Guided Endonuclease technology for
genome engineering. There are two distinct components to this system: (1) a
guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas)
nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by
the base-pairing between the gRNA sequence and the complement to the target
sequence in the genomic DNA. For successful binding of Cas9, the genomic
target sequence must also contain the correct Protospacer Adjacent Motiff
(PAM) sequence immediately following the target sequence.
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包装清单：
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产品编号
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产品名称
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包装
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XY0123
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CRISPR-Cas9
Mouse Monoclonal Antibody
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50μl
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AZ050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件：
CRISPR-Cas9
Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10