Akt1 Mouse Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0045
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Akt1 Mouse
Monoclonal Antibody
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50μl
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416.00元
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来源
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用途
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交叉反应性
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分子量
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Mouse
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WB, FC, IHC,
ICC, IF
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H, M
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56KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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1:50-1:100
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1:50-1:100
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1:50-1:100
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Akt1 Mouse
Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgG2b
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with a synthetic peptide (KLH-coupled)
corresponding to a region of AKT1.
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Gene ID
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207
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SwissProt
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P31749/P31751/Q9Y243
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Synonyms
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RAC-PK-alpha;
Protein kinase B; PKB;C-AKT;RAC-PK-beta; Protein kinase Akt-2; Protein kinase
B, beta; RAC-PK-gamma; Protein kinase Akt-3; Protein kinase B, gamma; STK-2
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Category
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PI3K / Akt
Signaling
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Background
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The
serine-threonine protein kinase AKT1 is catalytically inactive in
serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2
are activated by platelet-derived growth factor. The activation is rapid and
specific, and it is abrogated by mutations in the pleckstrin homology domain
of AKT1. It was shown that the activation occurs through phosphatidylinositol
3-kinase. In the developing nervous system AKT is a critical mediator of
growth factor-induced neuronal survival. Survival factors can suppress
apoptosis in a transcription-independent manner by activating the
serine/threonine kinase AKT1, which then phosphorylates and inactivates
components of the apoptotic machinery. Mice lacking Akt1 display a 25%
reduction in body mass, indicating that Akt1 is critical for transmitting
growth-promoting signals, most likely via the igf1 receptor. Mice lacking
Akt1 are also resistant to cancer: They experience considerable delay in
tumor growth initiated by the large T antigen or the Neuoncogene.
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包装清单：
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产品编号
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产品名称
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包装
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XY0045
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Akt1 Mouse
Monoclonal Antibody
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50μl
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AZ050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件：
Akt1 Mouse
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于**荧光(IF)、**组化(IHC)、**细胞化学(ICC)等适当用途。如果希望获得*佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。