Recombinant Human Nesfatin-1
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产品名称: Recombinant Human Nesfatin-1
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human Nesfatin-1由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human Nesfatin-1所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human Nesfatin-1
的详细介绍
Recombinant Human Nesfatin-1
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产品编号
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产品名称
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产品包装
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产品价格
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XY7221-1mg
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Recombinant
Human Nesfatin-1
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1mg
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5665.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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4925
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P80303
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E. coli
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82aa
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9.6kDa
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－
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About this
protein
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Name
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Recombinant
Human Nesfatin-1 (Recombinant Human Nesfatin; rHuNesfatin-1)；重组人Nesfatin-1蛋白
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Synonyms
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NEFA; NUCB2;
Nucleobindin-2; DNA-binding protein NEFA; NEFAGastric cancer antigen Zg4;
nucleobindin 2; nucleobindin-2; nucleobinding 2
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The biological activity is
tested by in vivo assay using healthy wild type male mice (C57BL/6J).
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuNesfatin-1 as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Others
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Background
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Nesfatin is a
metabolic polypeptide and is the N-terminal region of the precursor protein,
Nucleobindin2 (encoded by NUCB2 gene). It is a naturally occurring protein
and originally identified as a hypothalamic neuropeptide. Additionally,
Nesfatin can be found in other areas of brain, and in pancreatic isletsβ-cells, gastric endocrine cells and adipocytes. It is responsible
for regulating appetite and production of body fat. Excess nesfatin-1 in the
brain leads to a loss of appetite, less frequent hunger, a "sense of
fullness", and a drop in body fat and weight. A lack of nesfatin-1 in
the brain leads to an increase of appetite, more frequent episodes of hunger,
an increase of body fat and weight, and the inability to "feel
full".
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Amino Acid
Sequence
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VPIDIDKTKV QNIHPVESAK IEPPDTGLYY
DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL
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包装清单:
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产品编号
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产品名称
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包装
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XY7221-20μg
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Recombinant
Human Nesfatin-1
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20μg
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XY7221-100μg
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Recombinant
Human Nesfatin-1
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100μg
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XY7221-1mg
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Recombinant
Human Nesfatin-1
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。