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产品名称: Recombinant Human UBE2M
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human UBE2M由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human UBE2M所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human UBE2M
的详细介绍
Recombinant Human UBE2M
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产品编号
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产品名称
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产品包装
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产品价格
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XY7127-1mg
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Recombinant
Human UBE2M
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1mg
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12354.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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9040
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P61081
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E. coli
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183aa
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20.9kDa
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－
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About this
Antibody
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Name
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Recombinant
Human UBE2M (Recombinant Human Ubiquitin-conjugating Enzyme E2 M; rHuUBE2M)；重组人泛素结合酶E2M
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Synonyms
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UBC12; UbcH12;
UBC-RS2; EC 6.3.2; EC 6.3.2.-; hUbc12; NEDD8 carrier protein; NEDD8 protein
ligase; UBC12NEDD8-conjugating enzyme Ubc12; UBC-RS2; ubiquitin carrier
protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme
E2M (homologous to yeast UBC12); ubiquitin-conjugating enzyme E2M (UBC12
homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
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Purity
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>95% by SDS-PAGE
and HPLC analyses.
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Biological
Activity
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Data is not
available.
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Physical
Appearance
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Sterile
Filtered Liquid.
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Formulation
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A 0.2μm filtered concentrated solution in 50mM HEPES, pH6.5, with 125mM
NaCl, 10% Glycerol, 5% Trehalose, 1mM DTT.
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Endotoxin
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Less than
0.1EU/μg of rHuUBE2M as determined by LAL method.
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Reconstitution
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N/A
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Category
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Ubiquitins
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Background
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NEDD8-Conjugating
Enzyme Ubc12 belongs to the ubiquitin-conjugating enzyme family and is
encoded by the UBE2M gene in humans. The ubiquitin-conjugating enzymes, also
known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part
in the second step in the ubiquitination reaction. In this reaction, E1
activates the ubiquitin by covalently attaching the molecule to its active
site cysteine residue. The activated ubiquitin is then transferred to an E2
cysteine and then the E2 molecule binds E3 via a structurally conserved
binding region. The UBC12 accepts the ubiquitin-like protein NEDD8 from the
UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests
that the RBX1-UBE2M complex neddylates specific target proteins, such as
CUL1, CUL2, CUL3 and CUL4. Additionally, it involved in cell proliferation.
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Amino Acid
Sequence
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MIKLFSLKQQ KKEEESAGGT KGSSKKASAA
QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY
HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG
GYIGSTYFER CLK
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包装清单:
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产品编号
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产品名称
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包装
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XY7127-5μg
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Recombinant
Human UBE2M
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5μg
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XY7127-20μg
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Recombinant
Human UBE2M
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20μg
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XY7127-100μg
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Recombinant
Human UBE2M
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100μg
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XY7127-1mg
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Recombinant
Human UBE2M
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。