Recombinant β-lactamase TEM-1
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产品名称: Recombinant β-lactamase TEM-1
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant β-lactamase TEM-1由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant β-lactamase TEM-1所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant β-lactamase TEM-1
的详细介绍
Recombinant β-lactamase TEM-1
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产品编号
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产品名称
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产品包装
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产品价格
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XY7082-100mg
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Recombinant β-lactamase TEM-1
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100mg
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18666.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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－
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2716540
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P62593
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E. coli
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264aa
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28.9kDa
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－
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About this
Antibody
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Name
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Recombinant β-lactamase TEM-1 (Recombinant β-lactamase
TEM-1; rTEM-1)；重组β-内酰胺酶TEM-1
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Synonyms
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Beta-lactamase
TEM; IRT-4; Penicillinase; TEM-1; TEM-16/CAZ-7; TEM-2; TEM-24/CAZ-6; TEM-3;
TEM-4; TEM-5; TEM-6; TEM-8/CAZ-2; bla; blaT-3; blaT-4; blaT-5; blaT-6
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Purity
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>95% by
SDS-PAGE.
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Biological
Activity
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Fully
biologically active when compared to standard. One unit of enzyme activity is
defined as the amount of enzyme which will hydrolyze 1.0μmol of benzyl penicillin in presence of EDTA at pH 7.0 and at 25℃.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in 100mM Tris, pH7.0.
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Endotoxin
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N/A
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Enzymes
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Background
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Beta-lactamases
are enzymes produced by some bacteria and are responsible for their
resistance to beta-lactam antibiotics like penicillins, cephamycins, and
carbapenems. The lactamase enzyme breaks the β-lactam ring open
and deactivates the molecule's antibacterial properties because of a common
element in these antibiotics molecular structure: a four-atom ring known as a
betalactam. TEM-1 is the most commonly-encountered beta-lactamase in
gram-negative bacteria. Up to 90% of ampicillin resistance in E. coli is due to the
production of TEM-1. Also responsible for the ampicillin and penicillin
resistance that is seen in H.influenzae and N.gonorrhoeae in increasing
numbers. Based upon different combinations of changes, currently 140 TEM-type
enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264
amino acids residues.
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Amino Acid
Sequence
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MHPETLVKVK DAEDQLGARV GYIELDLNSG
KILESFRPEE RFPMMSTFKV LLCGAVLSRV DAGQEQLGRR IHYSQNDLVE YSPVTEKHLT DGMTVRELCS
AAITMSDNTA ANLLLTTIGG PKELTAFLHN MGDHVTRLDR WEPELNEAIP NDERDTTMPA AMATTLRKLL
TGELLTLASR QQLIDWMEAD KVAGPLLRSA LPAGWFIADK SGAGERGSRG IIAALGPDGK PSRIVVIYTT
GSQATMDERN RQIAEIGASL IKHW
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包装清单:
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产品编号
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产品名称
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包装
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XY7082-1mg
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Recombinant β-lactamase TEM-1
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1mg
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XY7082-5mg
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Recombinant β-lactamase TEM-1
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5mg
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XY7082-10mg
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Recombinant β-lactamase TEM-1
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10mg
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XY7082-100mg
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Recombinant β-lactamase TEM-1
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100mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。