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产品名称: Recombinant Human Leptin
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human Leptin由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human Leptin所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human Leptin
的详细介绍
Recombinant Human Leptin
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产品编号
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产品名称
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产品包装
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产品价格
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XY6969-5mg
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Recombinant
Human Leptin
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5mg
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2601.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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3952
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P41159
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E. coli
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146aa
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16kDa
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－
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About this
Protein
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Name
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Recombinant
Human Leptin (Recombinant Human Leptin; rHuLeptin)；重组人瘦素
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Synonyms
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LEP; OB;
FLJ94114; leptin; leptin (murine obesity homolog); leptin (obesity homolog,
mouse); Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS
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Purity
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>97% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells
is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuLeptin as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Hormones
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Background
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Human Leptin
plays a key role in regulating energy intake and energy expenditure,
including appetite and metabolism. It is one of the most important adipose
derived hormones. The Ob (Lep) gene (Ob for obese, Lep for leptin) is located
on chromosome 7 in humans. The protein is manufactured primarily in the
adipocytes of white adipose tissue, and the level of circulating leptin is
directly proportional to the total amount of fat in the body. Leptin acts on
receptors in the hypothalamus of the brain where it inhibits appetite by (1)
counteracting the effects of neuropeptide Y (a potent feeding stimulant
secreted by cells in the gut and in the hypothalamus); (2) counteracting the
effects of anandamide (another potent feeding stimulant that binds to the
same receptors as THC), and (3) promoting the synthesis of α-MSH, an appetite suppressant. This appetite inhibition is
long-term, in contrast to the rapid inhibition of eating by cholecystokinin
(CCK) and the slower suppression of hunger between meals mediated by PYY3-36.
The absence of leptin (or its receptor) leads to uncontrolled food intake and
resulting obesity.
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Amino Acid
Sequence
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VPIQKVQDDT KTLIKTIVTR INDISHTQSV
SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW
ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
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包装清单:
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产品编号
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产品名称
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包装
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XY6969-200μg
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Recombinant
Human Leptin
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200μg
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XY6969-1mg
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Recombinant
Human Leptin
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1mg
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XY6969-5mg
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Recombinant
Human Leptin
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5mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。