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产品名称: Recombinant Rat Midkine
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Rat Midkine由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Rat Midkine所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Rat Midkine
的详细介绍
Recombinant Rat Midkine
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产品编号
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产品名称
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产品包装
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产品价格
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XY6425-1mg
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Recombinant
Rat Midkine
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1mg
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15611.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Rat
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81517
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Q9R1S9
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E. coli
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120aa
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13.2kDa
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－
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About this
Antibody
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Name
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Recombinant
Rat Midkine (Recombinant Rat Midkine; rRtMidkine)；重组大鼠中期因子
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Synonyms
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ARAP; MDK;
MEK; MK1; NEGF2; Amphiregulin-associated protein; Midgestation and kidney
protein; midkine; midkine (neurite growth-promoting factor 2); MK1; MKARAP;
NEGF2FLJ27379; Neurite outgrowth-promoting factor 2; Neurite
outgrowth-promoting protein
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Purity
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>97% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The biological activity
determined by a chemotaxis bioassay using human neutrophils is in a
concentration range of 10-100ng/ml.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than
0.1EU/μg of rRtMidkine as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Midkine, also
named MK, MK1, NEGF 2, is belonging to the neurotrophic and
developmentally-regulated heparin-binding molecules family and it is encoded
by the MDK gene. Midkine includes five intrachain disulfide bonds which held
two domains and there are three antiparallel beta-sheets in each domain. A
chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta
(PTPzeta), is a receptor for MK. MK promotes the growth, survival, and
migration of various cells, and plays roles in neurogenesis and epithelial
mesenchymal interactions during organogenesis. The predicted molecular weight
is approximately 13.2kDa, based on a mature peptide length of 118 amino acid
residues in the mouse and 121 amino acid residues in the human. Mature rat
midkine shares 99% and 91%a.a. sequence identity with human and murine
midkine, respectively.
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Amino Acid
Sequence
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VAKKKDKVKK GSECSEWTWG PCTPSSKDCG
MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT
KPCTSKTKSK AKAKKGKGKD
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包装清单:
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产品编号
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产品名称
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包装
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XY6425-5μg
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Recombinant
Rat Midkine
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5μg
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XY6425-20μg
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Recombinant
Rat Midkine
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20μg
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XY6425-100μg
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Recombinant
Rat Midkine
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100μg
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XY6425-1mg
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Recombinant
Rat Midkine
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。