Recombinant Human EMAP-II
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产品名称: Recombinant Human EMAP-II
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human EMAP-II由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human EMAP-II所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human EMAP-II
的详细介绍
Recombinant Human EMAP-II
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产品编号
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产品名称
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产品包装
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产品价格
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XY381-1mg
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Recombinant
Human EMAP-II
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1mg
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20294.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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9255
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Q12904
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E. coli
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166aa
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18.2kDa
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－
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About this
Antibody
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Name
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Recombinant
Human EMAP-II (Recombinant Human Endothelial-Monocyte Activating Polypeptide
II; rHuEMAP-II)；重组人内皮单核细胞活化多肽II
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Synonyms
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AIMP1; SCYE1;
aminoacyl tRNA synthetase complex-interacting multifunctional protein 1;
EMAP-2; EMAPIImember 1 (endothelialmonocyte-activating); endothelial-monocyte
activating polypeptide II; Multisynthase complex auxiliary component p43;
multisynthetase complex auxiliary component p43
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Purity
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>98% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by the
apoptotic effect using serum free human MCF-7 cells is less than 40ng/ml,
corresponding to a specific activity of >2.5×104IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuEMAP-II as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Endothelial-Monocyte
Activating Polypeptide II (EMAP-II) is a tumor derived cytokine that exerts a
wide range of activities on endothelial cells, monocytes and neutrophils.
EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel
formation, and can induce apoptosis. It is also chemotactic towards
neutrophils and monocytes and induces myeloperoxidase activity from
neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of
vascular beds and suppresses the growth of primary and secondary tumors
without affecting normal tissues. Mature EMAP-II is an 18.3kDa protein, which
is synthesized as the C-terminal portion of a biologically inactive precursor
protein containing a propeptide of 146 amino acid residues.
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Amino Acid
Sequence
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SKPIDVSRLD LRIGCIITAR KHPDADSLYV
EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV
PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK
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包装清单:
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产品编号
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产品名称
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包装
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XY381-5μg
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Recombinant
Human EMAP-II
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5μg
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XY381-20μg
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Recombinant
Human EMAP-II
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20μg
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XY381-100μg
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Recombinant
Human EMAP-II
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100μg
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XY381-1mg
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Recombinant
Human EMAP-II
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。