如果您对该产品感兴趣的话,可以
产品名称: Recombinant Human M-CSF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human M-CSF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human M-CSF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human M-CSF
的详细介绍
Recombinant Human M-CSF
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY313-1mg
|
Recombinant
Human M-CSF
|
1mg
|
22133.00元
|
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
1435
|
P09603
|
E. coli
|
158aa
|
36.8kDa
|
－
|
|
About this
protein
|
|
Name
|
Recombinant
Human M-CSF (Recombinant Human Macrophage Colony Stimulating Factor;
rHuM-CSF)；重组人巨噬细胞集落刺激因子
|
|
Synonyms
|
CSF1; CSF-1;
colony stimulating factor 1 (macrophage); CSF-1; Lanimostim; macrophage
colony stimulating factor; macrophage colony-stimulating factor 1; M-CSF;
MCSFlanimostim; MGC31930
|
|
Purity
|
>95% by
SDS-PAGE and HPLC analyses.
|
|
Biological
Activity
|
Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine M-NFS-60 cells is less than 1ng/ml,
corresponding to a specific activity of >1.0×106IU/mg.
|
|
Physical
Appearance
|
Sterile
Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized
from a 0.2μm filtered solution in PBS, pH7.4.
|
|
Endotoxin
|
Less than 1EU/μg of rHuM-CSF as determined by LAL method.
|
|
Reconstitution
|
We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water to a concentration of
0.1mg/ml. Stock solutions should be apportioned into working aliquots and
stored at ≤-20℃. Further dilutions should
be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Macrophage
Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic
growth factor that is involved in the proliferation, differentiation, and
survival of monocytes, macrophages, and bone marrow progenitor cells. It is
produced by osteoblasts (as a result of endocrine stimulation by parathyroid
hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R.
M-CSF is a four α-helical bundle cytokine and its active form is found
extracellularly as a disulfide-linked homodimer. Four transcript variants
encoding three different isoforms have been reported for M-CSF gene. Although
forms may vary, all of them contain the N-terminal 150a.a. portion that is
necessary and sufficient for interaction with the receptor. The first 223a.a.
of mature human M-CSF shares 88%, 86%, 81% and 74% sequence identity with
corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human
M-CSF is active in the mouse, but mouse M-CSF is reported to be
species-specific.
|
|
Amino Acid
Sequence
|
EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS
CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK
ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY313-2μg
|
Recombinant
Human M-CSF
|
2μg
|
|
XY313-10μg
|
Recombinant
Human M-CSF
|
10μg
|
|
XY313-100μg
|
Recombinant
Human M-CSF
|
100μg
|
|
XY313-1mg
|
Recombinant
Human M-CSF
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。