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产品名称: Recombinant Human IL-12
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human IL-12由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human IL-12所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human IL-12
的详细介绍
Recombinant Human IL-12
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产品编号
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产品名称
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产品包装
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产品价格
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XY174-1mg
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Recombinant
Human IL-12
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1mg
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40588.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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3592
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P29459
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CHO
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503aa
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75kDa
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－
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About this
Protein
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Name
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Recombinant
Human IL-12 (Recombinant Human Interleukin-12; rHuIL-12)；重组人白细胞介素12
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Synonyms
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CLMF1; IL12A;
NKSF1; CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12
subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural
killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1,
p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural
killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor
chain 1; NK cell stimulatory factor chain 1; NKSF1; p35; p35
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using PHA-activated human T lymphoblasts is less
than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuIL-12 as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Interleukin-12
(IL-12), also known as NKSF or CLMF, is a pleiotropic cytokine originally
identified in the medium of activated human B lymphoblastoid cell lines. The
p40 subunit of IL-12 has been shown to have extensive amino acid sequence
homology to the extracellular domain of the human IL-6 receptor while the p35
subunit shows distant but significant sequence similarity to IL-6, G-CSF, and
chicken MGF. These observations have led to the suggestion that IL-12 might
have evolved from a cytokine/soluble receptor complex. Human and murine IL-12
share 70% and 60% amino acid sequence homology in their p40 and p35 subunits,
respectively. IL-12 apparently shows species specificity with human IL-12
reportedly showing minimal activity in the murine system.
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Amino Acid
Sequence
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p35 Subunit: RNLPVATPDP GMFPCLHHSQ
NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA
SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS
LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP
GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW
STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG
DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV
EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW
ASVPCS
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包装清单:
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产品编号
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产品名称
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包装
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XY174-2μg
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Recombinant
Human IL-12
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2μg
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XY174-10μg
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Recombinant
Human IL-12
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10μg
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XY174-1mg
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Recombinant
Human IL-12
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。