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产品名称: Recombinant Human IL-3
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human IL-3由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human IL-3所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human IL-3
的详细介绍
Recombinant Human IL-3
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产品编号
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产品名称
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产品包装
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产品价格
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XY124-1mg
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Recombinant
Human IL-3
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1mg
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15444.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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3562
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P08700
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E. coli
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133aa
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15kDa
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－
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About this
protein
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Name
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Recombinant
Human IL-3 (Recombinant Human Interleukin-3; rHuIL-3)；重组人白细胞介素3
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Synonyms
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Hematopoietic
growth factor; IL-3MGC79398; interleukin 3 (colony-stimulating factor,
multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor;
MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor;
Multipotential colony-stimulating factor; P-cell stimulating factor;
P-cell-stimulating factor
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Purity
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>96% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using human TF-1 cells is less than 0.1ng/ml,
corresponding to a specific activity of >1.0×107IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuIL-3 as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Interleukin-3
(IL-3) is an interleukin, a type of biological signal (cytokine) which is
encoded by the IL-3 gene located on chromosome 5 and produced primarily by
activated T cells beside human thymic epithelial cells, activated murine mast
cells, murine keratinocytes and neurons/astrocytes. The protein acts in
hematopoiesis by controlling the production, differentiation, and function of
2 related white cell populations of the blood, the granulocytes and the
monocytes-macrophages. The human IL-3 reported to be a monomer, as it is
known, contains 133 amino acids residues which is a single non-glycosylated
polypeptide. Specifically, human and murine IL-3 share low homology and it
does not show activity on murine cells.
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Amino Acid
Sequence
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APMTQTTSLK TSWVNCSNMI DEIITHLKQP
PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK
DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF
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包装清单:
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产品编号
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产品名称
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包装
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XY124-2μg
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Recombinant
Human IL-3
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2μg
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XY124-10μg
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Recombinant
Human IL-3
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10μg
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XY124-100μg
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Recombinant
Human IL-3
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100μg
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XY124-1mg
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Recombinant
Human IL-3
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。