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产品名称: Recombinant Protein A
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Protein A由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。Recombinant Protein A而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Protein A
的详细介绍
Recombinant Protein A
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产品编号
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产品名称
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产品包装
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产品价格
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XY5016-1g
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Recombinant
Protein A
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1g
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5218.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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-
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-
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-
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E. coli
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296aa
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33.4kDa
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-
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About this
Protein
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Name
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Recombinant
Protein A (Recombinant Protein A; Protein A);重组蛋白A
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Synonyms
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N/A
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Purity
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>97% by
SDS-PAGE and HPLC analyses
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Biological
Activity
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The
recombinant Protein A is a genetically engineered protein containing 5
IgG-binding regions of protein A. Cell wall binding region, cell membrane
binding region and albumin binding region have been removed from the
recombinant Protein A to ensure the maximum specific IgG binding. The
recombinant Protein A is ideal for purification of polyclonal or monoclonal
IgG antibodies. Protein A binds to various human, mouse and rat IgG
subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat
IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from additive free solution.
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Endotoxin
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Less than
0.1EU/μg of Protein A as determined by LAL method.
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Reconstitution
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Dissolve in
distilled water or saline.
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Category
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Protein A
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Background
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Protein A is a
cell wall component produced by several strains of Staphylococcus aureus that
consists of a single polypeptide chain and contains little or no
carbohydrate. Recombinant Protein A is produced in E. Coli and
functions essentially the same as native Protein A. It consists of 5
IgG-binding domains E-D-A-B-C aligned in series.
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Amino Acid
Sequence
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NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF
IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS
QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL
NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF
NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEED
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包装清单:
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产品编号
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产品名称
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包装
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XY5016-10mg
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Recombinant
Protein A
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10mg
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XY5016-100mg
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Recombinant
Protein A
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100mg
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XY5016-1g
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Recombinant
Protein A
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1g
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
为了您的**和健康,请穿实验服并戴一次性手套操作。