pABS018是一个植物细胞CAS9和gRNA表达质粒。This cloning procedure is very rapid, since the AarI enzyme cuts outside of its recognition site, the overhangs are incompatible and the subsequently-cloned in fragment does not restore the AarI site. Therefore, no purification is required to remove the restriction enzyme prior to ligation. In my experience, more than half the clones contain the desired insert.