质粒序列
LOCUS Exported 7193 bp ds-DNA circular SYN 12-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 14
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7193)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 12, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7193
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 5802..6346
/direction=RIGHT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
promoter 6872..6974
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 6975..441
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaag aatagttacg
541 gcttatgaca tctttgtgga cacatcattc actttttatt cacatccggc cctgaactcg
601 ctaggacttg ccccggtgca ttttttaaat acccgcgaaa aatagagctg atcgtcaaat
661 ccaacattgc gcccaacggt cgctatcggc attcgcgtag tgctaagcag aagtttcgcc
721 tggctgatac gctgatcttc gcgccagctc aatacgctaa tgcctaactg ctggcggaac
781 agatgtgata accgggaggg cgacaggcag acatgctggg cgacgctggc gatatcaaaa
841 tggctgtccg ccagatggtc gctgatatac tggcaggcat cgcgcacacg gctatccatc
901 ggcgggtgca acgactcatt aattaccgcc atacgtctga gcaacaactg ctccagcaga
961 ttgatcgcca gtagctcaga atagcgacct tccccttgcc cggcgctgat gatctgcccg
1021 aacagttcgc tgaaatgcgg ctggcgcgcc tcgtccgggc ggaaaaatcc tgtctgggca
1081 aagattgtcg gccaggtcag ccactcctgc cagtaggcgc gaggccggaa ataaacccac
1141 tggtgatacc actcgctggc gtccggatgc cgtccatagt gatgaatctc gcccggcgga
1201 aacaataata tatcgccagg ccgacagaca aactgctcgc cattattatt aatgacgccc
1261 tctccgcgga tggtcaggtt aagaatatat cccttcatgc ccaacggacg atcgataaaa
1321 aaatccagat atccattcgc ttcaattggc gtcagcccgg cgaccagatg ggcattaaat
1381 gaatatcccg gcaatagcgg atcattttgc gtttcagcca tgatttctct accccccgat
1441 gttcagagaa gaaccaaatt gtccatatcg accaggacga cagagcttcc gtctccgcaa
1501 gactttgcgc ttgatgaaag cacgtatcaa ccccgcttgt gaaaagcgct ttgtaacaaa
1561 agcgtacagt tcaggcgata aaattaagta acagaagtgt ctataactat ggctggaatg
1621 tccacattga atatttgcac agcgtcacac tttgcaaagc attagcattt ttgtccataa
1681 gattagcgga tcctgcctga cggtttttgc cgcgactctc tataatttct ccatacctgt
1741 ttttctggat ggagtaagac catggctatg gattgtggag acgtttagat gggtaaaata
1801 attggtatcg acctgggtac taccaactct tgtgtagcga ttatggatgg caccactcct
1861 cgcgtgctgg agaacgccga aggcgatcgc accacgcctt ctatcattgc ctatacccag
1921 gatggtgaaa ctctagttgg tcagccggct aaacgtcagg cagtgacgaa cccgcaaaac
1981 actctgtttg cgattaaacg cctgattggt cgccgcttcc aggacgaaga agtacagcgt
2041 gatgtttcca tcatgccgtt caaaattatt gctgctgata acggcgacgc atgggtcgaa
2101 gttaaaggcc agaaaatggc accgccgcag atttctgctg aagtgctgaa aaaaatgaag
2161 aaaaccgctg aagattacct gggtgaaccg gtaactgaag ctgttatcac cgtaccggca
2221 tactttaacg atgctcagcg tcaggcaacc aaagacgcag gccgtatcgc tggtctggaa
2281 gtaaaacgta tcatcaacga accgaccgca gctgcgctgg cttacggtct ggacaaaggc
2341 actggcaacc gtactatcgc ggtttatgac ctgggtggtg gtactttcga tatttctatt
2401 atcgaaatcg acgaagttga cggcgaaaaa accttcgaag ttctggcaac caacggtgat
2461 acccacctgg ggggtgaaga cttcgacagc cgtctgatca actatctggt tgaagaattc
2521 aagaaagatc agggcattga cctgcgcaac gatccgctgg caatgcagcg cctgaaagaa
2581 gcggcagaaa aagcgaaaat cgaactgtct tccgctcagc agaccgacgt taacctgcca
2641 tacatcactg cagacgcgac cggtccgaaa cacatgaaca tcaaagtgac tcgtgcgaaa
2701 ctggaaagcc tggttgaaga tctggtaaac cgttccattg agccgctgaa agttgcactg
2761 caggacgctg gcctgtccgt atctgatatc gacgacgtta tcctcgttgg tggtcagact
2821 cgtatgccaa tggttcagaa gaaagttgct gagttctttg gtaaagagcc gcgtaaagac
2881 gttaacccgg acgaagctgt agcaatcggt gctgctgttc agggtggtgt tctgactggt
2941 gacgtaaaag acgtactgct gctggacgtt accccgctgt ctctgggtat cgaaaccatg
3001 ggcggtgtga tgacgacgct gatcgcgaaa aacaccacta tcccgaccaa gcacagccag
3061 gtgttctcta ccgctgaaga caaccagtct gcggtaacca tccatgtgct gcagggtgaa
3121 cgtaaacgtg cggctgataa caaatctctg ggtcagttca acctagatgg tatcaacccg
3181 gcaccgcgcg gcatgccgca gatcgaagtt accttcgata tcgatgctga cggtatcctg
3241 cacgtttccg cgaaagataa aaacagcggt aaagagcaga agatcaccat caaggcttct
3301 tctggtctga acgaagatga aatccagaaa atggtacgcg acgcagaagc taacgccgaa
3361 gctgaccgta agtttgaaga gctggtacag actcgcaacc agggcgacca tctgctgcac
3421 agcacccgta agcaggttga agaagcaggc gacaaactgc cggctgacga caaaactgct
3481 atcgagtctg cgctgactgc actggaaact gctctgaaag gtgaagacaa agccgctatc
3541 gaagcgaaaa tgcaggaact ggcacaggtt tcccagaaac tgatggaaat cgcccagcag
3601 caacatgccc agcagcagac tgccggtgct gatgcttctg caaacaacgc gaaagatgac
3661 gatgttgtcg acgctgaatt tgaagaagtc aaagacaaaa aataatcgcc ctataaacgg
3721 gtaattatac tgacacgggc gaaggggaat ttcctccccg cccgtgcatt catctagggg
3781 caatttaaaa aagatggcta agcaagatta ttacgagatt ttaggcgttt ccaaaacagc
3841 ggaagagcgt gaaatcagaa aggcctacaa acgcctggcc atgaaatacc acccggaccg
3901 taaccagggt gacaaagagg ccgaggcgaa atttaaagag atcaaggaag cttatgaagt
3961 tctgaccgac tcgcaaaaac gtgcggcata cgatcagtat ggtcatgctg cgtttgagca
4021 aggtggcatg ggcggcggcg gttctggcgg cggcgcagac ttcagcgata tttttggtga
4081 cgttttcggc gatatttttg gcggcggacg tggtcgtcaa cgtgcggcgc gcggtgctga
4141 tttacgctat aacatggagc tcaccctcga agaagctgta cgtggcgtga ccaaagagat
4201 ccgcattccg actctggaag agtgtgacgt ttgccacggt agcggtgcaa aaccaggtac
4261 acagccgcag acttgtccga cctgtcatgg ttctggtcag gtgcagatgc gccagggatt
4321 cttcgctgta cagcagacct gtccacactg tcagggccgc ggtacgctga tcaaagatcc
4381 gtgcaacaaa tgtcatggtc atggtcgtgt tgagcgcagc aaaacgctgt ccgttaaaat
4441 cccggcaggg gtggacactg gagaccgcat ccgtcttgcg ggcgaaggtg aagcgggcga
4501 gcatggcgca ccggcaggcg atctgtacgt tcaggttcag gttaaacagc acccgatttt
4561 cgagcgtgaa ggcaacaacc tgtattgcga agtcccgatc aacttcgcta tggcggcgct
4621 gggtggcgaa atcgaagtac cgacccttga tggtcgcgtc aaactgaaag tgcctggcga
4681 aacccagacc ggtaagctat tccgtatgcg cggtaaaggc gtcaagtctg tccgcggtgg
4741 cgcgcagggt gatttgctgt gccgcgttgt cgtcgaaaca ccggtaggcc tgaacgaaag
4801 gcagaaacag ctgctgcaag agctgcaaga aagcttcggt ggcccaaccg gcgagcacaa
4861 cagcccgcgc tcaaagagct tctttgatgg tgtgaagaag ttttttgacg acctgacccg
4921 ctaacctcgc ggagaaattc atgagtagta aagaacagaa aacgcctgag gggcaagccc
4981 cggaagaaat tatcatggat cagcacgaag agattgaggc agttgagcca gaagcttctg
5041 ctgagcaggt ggatccgcgc gatgaaaaag ttgcgaatct cgaagctcag ctggctgaag
5101 cccagacccg tgaacgtgac ggcattttgc gtgtaaaagc cgaaatggaa aacctgcgtc
5161 gtcgtactga actggatatt gaaaaagccc acaaattcgc gctggagaaa ttcatcaacg
5221 aattgctgcc ggtgattgat agcctggatc gtgcgctgga agtggctgat aaagctaacc
5281 cggatatgtc tgcgatggtt gaaggcattg agctgacgct gaagtcgatg ctggatgttg
5341 tgcgtaagtt tggcgttgaa gtgatcgccg aaactaacgt cccactggac ccgaatgtgc
5401 atcaggccat cgcaatggtg gaatctgatg acgttgcgcc aggtaacgta ctgggcatta
5461 tgcagaaggg ttatacgctg aatggtcgta cgattcgtgc ggcgatggtt actgtagcga
5521 aagcaaaagc ttaatttctg ctttcgtaat aattcacggc cctgcatgca agctcagatc
5581 tgagcttggc tgttttggcg gatgagagaa gaggtcgact ctagaggatc tactagtcat
5641 aggcgccgat atcagatccg gtaccaagct tatcgatgat aagctgtcaa acatgagaat
5701 tacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact
5761 gaaatctaga aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg
5821 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt
5881 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
5941 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca
6001 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg
6061 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
6121 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
6181 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
6241 cgccagggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
6301 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc
6361 ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt
6421 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
6481 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc
6541 ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac
6601 actccgctag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc
6661 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca
6721 tacactaaat cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
6781 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
6841 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
6901 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
6961 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
7021 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
7081 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
7141 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttc
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