质粒序列
LOCUS Exported 3442 bp ds-DNA circular SYN 03-DEC-2013
DEFINITION Bacterial vector for expressing N-terminally 6xHis-tagged proteins.
For other reading frames, use pQE-9 or pQE-11.
ACCESSION .
VERSION .
KEYWORDS pQE-10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3442)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..3442
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
misc_feature 147..172
/note="MCS"
/note="multiple cloning site"
misc_feature 172..182
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 188..282
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 326..985
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1050..1136
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(1618..2206)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2377..3237)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3238..3342)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatctcacc atcaccatca ccatacggat ccgtcgacct gcagccaagc ttaattagct
181 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
241 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgag
301 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
361 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
421 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
481 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
541 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
601 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
661 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
721 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
781 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
841 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
901 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
961 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
1021 tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact
1081 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
1141 cgctctagag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
1201 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
1261 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
1321 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
1381 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
1441 cgcttcctcg ctcactgact cgctgcgctc ggtctgtcgg ctgcggcgag cggtatcagc
1501 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
1561 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
1681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
1741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
1801 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
1861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
1921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
1981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2041 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
2401 acctatctca gcgatctgtc tatttcgttc atccatagct gcctgactcc ccgtcgtgta
2461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
2521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
2581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
2641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
2701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
2761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
2821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
2881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
2941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3361 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
3421 cacgaggccc tttcgtcttc ac
//