质粒序列
LOCUS Exported 3441 bp ds-DNA circular SYN 03-DEC-2013
DEFINITION Bacterial vector for expressing N-terminally 6xHis-tagged proteins.
For other reading frames, use pQE-9 or pQE-10.
ACCESSION .
VERSION .
KEYWORDS pQE-11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3441)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..3441
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
misc_feature 146..171
/note="MCS"
/note="multiple cloning site"
misc_feature 171..181
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 187..281
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 325..984
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1049..1135
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(1617..2205)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2376..3236)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3237..3341)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatctcacc atcaccatca ccatgggatc cgtcgacctg cagccaagct taattagctg
181 agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
241 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag cttggcgaga
301 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
361 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
421 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
481 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
541 tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
601 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
661 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
721 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
781 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
841 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
901 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
961 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
1021 ggggtaatga ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg
1081 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
1141 gctctagagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
1201 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
1261 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
1321 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
1381 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
1441 gcttcctcgc tcactgactc gctgcgctcg gtctgtcggc tgcggcgagc ggtatcagct
1501 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
1561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
1621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
1681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
1741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
1801 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
1861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
1921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
1981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
2041 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
2101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
2161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
2221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
2281 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
2341 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
2401 cctatctcag cgatctgtct atttcgttca tccatagctg cctgactccc cgtcgtgtag
2461 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
2521 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
2581 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
2641 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
2701 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
2761 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
2821 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
2881 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
2941 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
3001 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
3061 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
3121 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
3181 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
3241 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
3301 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
3361 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
3421 acgaggccct ttcgtcttca c
//