质粒序列
LOCUS Exported 4003 bp ds-DNA circular SYN 05-DEC-2013
DEFINITION Bacterial vector for expressing DHFR fusion proteins with an
N-terminal 6xHis tag. For other reading frames after DHFR, using
pQE-14 or pQE-15.
ACCESSION .
VERSION .
KEYWORDS pQE-13
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4003)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..4003
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 157..714
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/note="DHFR"
/translation="MVRPLNSIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKK"
misc_feature 727..743
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 749..843
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 887..1546
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1611..1697
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2179..2767)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2938..3798)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3799..3903)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatcgcatc accatcacca tcacggatcc ggcatcatgg ttcgaccatt gaactcgatc
181 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc
241 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag
301 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta
361 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat
421 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt
481 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa
541 ccaggccacc ttagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt
601 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct
661 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaaggttcc
721 agatcttaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
781 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
841 aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
901 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
961 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
1021 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
1081 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
1141 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
1201 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
1261 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
1321 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
1381 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
1441 gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct
1501 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
1561 ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac
1621 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
1681 tcctgagtag gacaaatccg ccgctctaga gctgcctcgc gcgtttcggt gatgacggtg
1741 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
1801 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
1861 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
1921 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
1981 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtctgtcg
2041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
2101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
2161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
2281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
2341 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
2401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
2461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
2521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
2581 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
2641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
2701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
2761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
2821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
2881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
2941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagc
3001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
3061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
3121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
3181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
3241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
3301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
3361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
3421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
3481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
3541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
3601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
3661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
3721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
3781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
3841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
3901 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
3961 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//