质粒序列
LOCUS Exported 4031 bp ds-DNA circular SYN 05-DEC-2013
DEFINITION Bacterial vector for expressing DHFR fusion proteins with an
N-terminal 6xHis tag. For other reading frames, use pQE-40 or
pQE-42.
ACCESSION .
VERSION .
KEYWORDS pQE-41
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4031)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..4031
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 157..714
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/note="DHFR"
/translation="MVRPLNSIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKK"
misc_feature 720..761
/note="MCS"
/note="multiple cloning site"
misc_feature 761..771
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 777..871
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 915..1574
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1639..1725
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2207..2795)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2966..3826)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3827..3931)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatcgcatc accatcacca tcacggatcc ggcatcatgg ttcgaccatt gaactcgatc
181 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc
241 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag
301 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta
361 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat
421 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt
481 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa
541 ccaggccacc ttagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt
601 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct
661 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaaggttca
721 gatctgcatg cggtaccccg ggtcgacctg cagccaagct taattagctg agcttggact
781 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
841 ttgccgccgg gcgtttttta ttggtgagaa tccaagctag cttggcgaga ttttcaggag
901 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
961 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
1021 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
1081 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
1141 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
1201 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
1261 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
1321 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
1381 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
1441 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct
1501 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
1561 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga
1621 ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
1681 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctctagagc
1741 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
1801 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
1861 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
1921 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
1981 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
2041 tcactgactc gctgcgctcg gtctgtcggc tgcggcgagc ggtatcagct cactcaaagg
2101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
2161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
2221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
2281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
2341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
2401 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
2461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
2521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
2581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
2641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
2701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
2761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
2821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
2881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
2941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
3001 cgatctgtct atttcgttca tccatagctg cctgactccc cgtcgtgtag ataactacga
3061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
3121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
3181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
3241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
3301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
3361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
3421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
3481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
3541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
3601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
3661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
3721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
3781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
3841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
3901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
3961 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
4021 ttcgtcttca c
//