phospho-CAMK4(Thr196 + Thr200)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 6728R
英文名称phospho-CAMK4(Thr196 + Thr200)
中文名称磷酸化钙/钙调蛋白依赖性蛋白激酶4抗体
别 名CAMKIV(phospho T196 + T200); Brain Ca(2+) calmodulin dependent protein kinase type 4; Brain Ca(2+) calmodulin dependent protein kinase type IV; Calcium / calmodulin dependent protein kinase type 4 catalytic chain; Calcium / calmodulin dependent protein kinase type IV catalytic chain; Calcium/calmodulin dependent protein kinase IV; Calcium/calmodulin dependent protein kinase type IV; CAM kinase 4; CAM kinase GR; CAM kinase IV; CaMK 4; CAMK GR; CaMK IV; CaMK4; CaMKGR; MGC36771.
说 明 书100ul
产品类型磷酸化抗体
研究领域细胞生物 神经生物学 信号转导 转录调节因子 激酶和磷酸酶 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
phospho-CAMK4(Thr196 + Thr200)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CAMKIV around the phosphorylation site of Thr196 + Thr200.:MK(p-T)VCG(p-T)PG
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-CAMK4(Thr196 + Thr200)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Ca2+/calmodulin dependent protein kinase type IV (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.
Function:
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consodilation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteolclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphrylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
Subunit:
Monomer (By similarity). Interacts with protein phosphatase 2A (PPP2CA/PPP2CB); the interaction is mutually exclusive with binding to Ca(2+)/calmodulin.
Subcellular Location:
Cytoplasm. Nucleus. Note=Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity).
Tissue Specificity:
Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
Post-translational modifications:
Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13.
Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation.
Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
SWISS:
Q16566
Gene ID:
814
phospho-CAMK4(Thr196 + Thr200)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-CAMK4(Thr196 + Thr200)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 IRS-2 胰岛素受体底物-2抗体
合格 UCP-2 线粒体脱偶连蛋白2抗体
合格 合格 SOCS3 细胞因子信号传导抑制蛋白3抗体
合格 合格 合格 CD44V6/HCAM CD44V6抗体
合格 BRN4 脑转录因子4蛋白抗体
合格 HTRA2 线粒体丝氨酸蛋白酶抗体
合格 PARP1 多腺苷二磷酸多聚酶抗体/多聚ADP-核糖聚合酶1抗体
合格 Retinoid X receptor alpha 核受体RXRα抗体
合格 Retinoid X receptor alpha 核受体RXRα抗体
合格 合格 合格 TERT 端粒酶逆转录酶抗体
合格 TERT 端粒酶逆转录酶抗体
合格 Caspase-9 活化半胱胺酸蛋白酶蛋白-9抗体
合格 RASSF1A Ras相关区域家族1A抗体
合格 FADD Fas死亡结构域相关蛋白
合格 CD19 CD19抗体
合格 CD19 CD19抗体
合格 PEDF 色素上皮源性因子抗体
合格 合格 PEDF 色素上皮源性因子抗体
合格 WNT4 信号通路Wnt4抗体
合格 CCR4 细胞表面趋化因子受体4抗体
合格 MDM2 双微体2癌基因抗体
合格 合格 CD14 内**受体抗体
合格 DCX/Doublecortin 双皮质素抗体
合格 DCX/Doublecortin 双皮质素抗体
合格 合格 SLC35D2 溶质载体家族蛋白35成员D2抗体
合格 合格 KRT81 细胞角蛋白81抗体
合格 RAG1AP1 RAG1激活蛋白1抗体
合格 Perforin 穿孔素抗体
合格 合格 TRIM38 TRIM38蛋白抗体
合格 合格 ZP2 卵透明带糖蛋白2抗体
合格 ADAM2 去整合素样金属蛋白酶2抗体
合格 SLC26A6 溶质载体家族蛋白26成员6抗体
合格 TMEM120A 跨膜蛋白120A抗体
合格 合格 合格 CK5+CK6 细胞角蛋白5+6抗体
合格 PTPRN2 胰岛细胞自身抗原相关蛋白PTPRN2抗体
合格 SPINK13 丝氨酸蛋白酶抑制剂SPINK13抗体
合格 DcR1 肿瘤坏死因子配体超家族成员10C
合格 CCL27 皮肤T细胞虏获趋化因子抗体
合格 CYP4F3 细胞色素P450 F3抗体
合格 NHEDC1 钠/氢交换样结构域蛋白1抗体