phospho-CASC3(Tyr181)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5283R
英文名称phospho-CASC3(Tyr181)
中文名称磷酸化肿瘤易感候选基因3抗体
别 名Barentsz protein; Btz; Cancer susceptibility candidate gene 3 protein; Metastatic lymph node protein 51; MLN 51 protein; MLN51; Protein barentsz; Protein CASC3; Protein MLN 51; CASC3_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学
抗体来源Rabbit
克隆类型Polyclonal
phospho-CASC3(Tyr181)抗体交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量76kDa
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CASC3 around the phosphorylation site of Tyr181:PA(p-Y)IP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-CASC3(Tyr181)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The multiprotein exon junction complex (EJC) is deposited on mRNAs upstream of exon–exon junctions as a consequence of pre-mRNA splicing. In mammalian cells, this complex serves as a key modulator of spliced mRNA metabolism. MLN51 is a nucleocytoplasmic shuttling protein that is overexpressed in breast cancer. The function of MLN51 in mammals remains elusive. Its fly homolog, named barentsz, as well as the proteins mago nashi and tsunagi have been shown to be required for proper oskar mRNA localization to the posterior pole of the oocyte. Magoh and Y14, the human homologs of mago nashi and tsunagi, are core components of the exon junction complex (EJC). The EJC is assembled on spliced mRNAs and plays important roles in post-splicing events including mRNA export, nonsense-mediated mRNA decay, and translation. Human MLN51 is an RNA-binding protein present in ribonucleo-protein complexes.
Function:
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons (By similarity). May play a role in mRNA transport (By similarity). Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer.
Subunit:
Forms homooligomers (By similarity). Interacts with STAU in an RNA-dependent manner (By similarity). Part of the EJC core complex that contains CASC3, EIF4A3, MAGOH and RBM8A. Found in a mRNA splicing-dependent exon junction complex (EJC), at least composed of ACIN1, CASC3, EIF4A3, MAGOH, PNN,
Subcellular Location:
Cytoplasm, perinuclear region. Nucleus. Nucleus speckle. Note=Predominantly found in the perinuclear region (By similarity). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Shuttles between the cytoplasm and the stress granules. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing. Colocalizes in nuclear speckles with MAGOH. Under stress condition, colocalizes with FMR1 and TIA1, but not MAGOH and RBM8A EJC core factors, in cytoplasmic stress granules, which contain stored mRNAs whose translation is stopped in response to stress.
Tissue Specificity:
Widely expressed. Overexpressed in breast cancers and metastasis, as well as in gastric cancers. [DOMAIN] The coiled coil domain may be involved in oligomerization.
Post-translational modifications:
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
Similarity:
Belongs to the CASC3 family.
SWISS:
O15234
Gene ID:
22794
phospho-CASC3(Tyr181)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-CASC3(Tyr181)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 phospho-beta Catenin (Ser33) 磷酸化β-连环蛋白/β-连环素/β链接素抗体
合格 Nestin 巢蛋白/神经上皮干细胞蛋白抗体
合格 eNOS 一氧化氮合成酶-3（内皮型）抗体
合格 合格 AQP9 水通道蛋白-9抗体
合格 PI 3 Kinase p85 alpha 磷脂酰肌醇激酶抗体
合格 Caspase-8 subunit p10 半胱氨酸蛋白酶8抗体
合格 Caspase-8 subunit p10 半胱氨酸蛋白酶8抗体
合格 NRXN3 轴突蛋白3抗体
合格 SNAIL Snail蛋白抗体
合格 Caspase-1 P10 天冬氨酸-胱氨酸特异性蛋白酶家族抗体
合格 N-cadherin N-钙粘附分子抗体
合格 合格 OPG 骨?；さ鞍?护骨素抗体
合格 Androgen receptor 雄**受体抗体
合格 Integrin beta 1/CD29 整合素β1/Integrin β1抗体
合格 Integrin beta 1/CD29 整合素β1/Integrin β1抗体
合格 合格 HMGB1 高迁移率族蛋白B1抗体
合格 C peptide (human) 人C-肽抗体
合格 合格 CD20 CD20抗体
合格 合格 CD20 CD20抗体
合格 合格 phospho-beta catenin (Tyr142) 磷酸化β 连环素蛋白抗体
合格 CD90 CD90抗体
合格 GPR30 G蛋白偶联受体30抗体
合格 GPR30 G蛋白偶联受体30抗体
合格 合格 PSD95 突触后密度蛋白95抗体
合格 PSD95 突触后密度蛋白95抗体
合格 MMP-1 基质金属蛋白酶-1抗体
合格 HSP90 beta 热休克蛋白90β/HSP90 β 抗体
合格 CDKN2A/p16-INK4a 抑癌基因p16抗体
合格 FSH receptor 促卵泡刺**受体抗体
合格 FSH receptor 促卵泡刺**受体抗体
合格 合格 NKG2D NK细胞受体2D抗体
合格 合格 NKG2D NK细胞受体2D抗体
合格 phospho-Fos B (Ser27) 磷酸化癌基因FOS蛋白B抗体
合格 合格 合格 HSP27 热休克蛋白27抗体
合格 SOD2 超氧化物歧化酶2抗体
合格 SOD2 超氧化物歧化酶2抗体
合格 DDIT3 GADD153抗体
合格 phospho-GSK-3 Beta(Ser9) 磷酸化糖原合酶激酶-3β抗体
合格 合格 DDIT3 GADD153抗体
合格 TGF alpha 转移生长因子α抗体