phospho-Caveolin-2 (Tyr19)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 7523R
英文名称phospho-Caveolin-2 (Tyr19)
中文名称磷酸化细胞质膜微囊蛋白-2抗体
别 名Caveolin 2 (phospho Y19); p-Caveolin 2(phospho Y19); CAV; CAV2; CAV2_HUMAN; Caveolae protein 20 kD; Caveolin 2; Caveolin 2 isoform a and b; Caveolin 2 isoform c; Caveolin-2; MGC12294; CAV2_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域心血管 细胞生物 信号转导 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
phospho-Caveolin-2 (Tyr19)抗体交叉反应 Mouse, Rat,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量18kDa
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from mouse Caveolin2 around the phosphorylation site of Tyr19.:DA(p-Y)SH
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Caveolin-2 (Tyr19)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Cellular localization: Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
Function:
May act as a scaffolding protein within caveolarmembranes. Interacts directly with G-protein alpha subunits and canfunctionally regulate their activity. Acts as an accessory proteinin conjunction with CAV1 in targeting to lipid rafts and drivingcaveolae formation. The Ser-36 phosphorylated form has a role inmodulating mitosis in endothelial cells. Positive regulator ofcellular mitogenesis of the MAPK signaling pathway. Required forthe insulin-stimulated nuclear translocation and activation ofMAPK1 and STAT3, and the subsequent regulation of cell cycleprogression (By similarity).
Subunit:
Monomer or homodimer. Interacts with CAV1; theinteraction forms a stable heterooligomeric complex that isrequired for targeting to lipid rafts and for caveolae formation.Tyrosine phosphorylated forms do not form heterooligomers with theTyr-19-phosphorylated form existing as a monomer or dimer, and theTyr-27-form as a monomer only. Interacts (tyrosine phosphorylatedform) with the SH2 domain-containing proteins, RASA1, NCK1 and SRC.Interacts (tyrosine phosphorylated form) with INSR, the interaction(Tyr-27-phosphorylated form) is increased on insulin stimulation.Interacts (Tyr-19 phosphorylated form) with MAPK1 (phosphorylatedform); the interaction, promoted by insulin, leads to nuclearlocation and MAPK1 activation. Interacts with STAT3; theinteraction is increased on insulin-induced tyrosinephosphorylation leading to STAT activation (By similarity).
Subcellular Location:
Nucleus. Cytoplasm. Golgi apparatusmembrane; Peripheral membrane protein. Cell membrane; Peripheralmembrane protein. Membrane, caveola; Peripheral membrane protein.Note=Potential hairpin-like structure in the membrane. Membraneprotein of caveolae. Tyr-19-phosphorylated form is enriched atsites of cell-cell contact and is translocated to the nucleus incomplex with MAPK1 in response to insulin (By similarity).Tyr-27-phosphorylated form is located both in the cytoplasm andplasma membrane. CAV1-mediated Ser-23-phosphorylated form locatesto the plasma membrane. Ser-36-phosphorylated form resides inintracellular compartments.
Tissue Specificity:
Expressed in endothelial cells, smooth musclecells, skeletal myoblasts and fibroblasts.
Post-translational modifications:
Phosphorylated on serine and tyrosine residues. CAV1 promotesphosphorylation on Ser-23 which then targets the complex to theplasma membrane, lipid rafts and caveolae. Phosphorylation onSer-36 appears to modulate mitosis in endothelial cells (Bysimilarity). Phosphorylation on both Tyr-19 and Tyr-27 is requiredfor insulin-induced 'Ser-727' phosphorylation of STAT3 and itsactivation. Phosphorylation on Tyr-19 is required forinsulin-induced phosphorylation of MAPK1 and DNA binding of STAT3.Tyrosine phosphorylation is induced by both EGF and insulin (Bysimilarity).
Similarity:
Belongs to the caveolin family.
SWISS:
Q9WVC3
Gene ID:
12390
phospho-Caveolin-2 (Tyr19)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Caveolin-2 (Tyr19)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 PRPF4 PRPF4蛋白抗体
合格 EGF 表皮生长因子抗体
合格 Angiotensin III 血管紧张素III抗体
合格 ADAM9 去整合素样金属蛋白酶9抗体
合格 EGF 表皮生长因子抗体
合格 RanBP3 RAN结合蛋白3抗体
合格 HDLBP 高密度脂蛋白结合蛋白抗体
合格 Collagen alpha-1(I) chain I型胶原蛋白
合格 MDH2 线粒体苹果酸脱氢酶2抗体
合格 SCN1A SCN1A抗体
合格 NIT2 NIT2蛋白抗体
合格 FAIM3 FAS凋亡抑制分子3抗体
合格 DLC1 肝癌缺失基因1抗体
合格 DLC2 肝癌缺失基因2抗体
合格 GSK3 alpha 糖原合酶激酶3α抗体
合格 BPNT1 二磷酸3核苷酸酶抗体
合格 phospho-Nrf2 (Ser40) 磷酸化核因子2相关因子2(Ser40)抗体
合格 SFRS9 丝氨酸/苏氨酸SFRS9抗体
合格 ZNF703 锌指蛋白703抗体
合格 FAM166A FAM166A蛋白抗体
合格 DNCLI1 胞质动力蛋白轻链中间体1抗体
合格 MVK 甲羟戊酸激酶抗体
合格 合格 PCDH7 原钙粘蛋白7抗体
合格 MVK 甲羟戊酸激酶抗体
合格 合格 NFKB p65 细胞核因子/k基因结合核因子抗体
合格 S100B S100B蛋白抗体
合格 NFKB p65 细胞核因子/k基因结合核因子抗体
合格 Bcl2A1 BCL2相关蛋白A1抗体
合格 HIP55 宫颈黏液蛋白相关蛋白抗体
合格 合格 合格 Integrin Alpha V + Beta 1 整合素αVβ1抗体
合格 MMP1 基质金属蛋白酶-1抗体
合格 GLUT1 葡萄糖转运蛋白1抗体
合格 LDB3 LIM结构域结合蛋白3抗体
合格 ADRB1 肾上腺素能受体β1抗体
合格 合格 SLC22A6 阴离子转运蛋白-1抗体
合格 PALM3 PALM3抗体
合格 CES2 羧酸酯酶2抗体
合格 合格 Dematin 红细胞膜带4.9蛋白抗体