phospho-c-Jun (Ser63)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3210R
英文名称phospho-c-Jun (Ser63)
中文名称磷酸化原癌基因c-Jun抗体
别 名c-Jun (phospho S63); c-Jun (phospho Ser63); p-c-Jun (Ser63); Transcription factor AP-1; Jun oncogene; JUN; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; JUN_HUMAN; Activator 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-c-Jun (Ser63)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human c-Jun around the phosphorylation site of Ser63:LT(p-S)PD
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-c-Jun (Ser63)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Function:
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.
Similarity:
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
SWISS:
P05412
Gene ID:
3725
phospho-c-Jun (Ser63)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-c-Jun (Ser63)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-EGFR (Tyr1068) 磷酸化表皮生长因子受体抗体
合格 ZNF8 锌指蛋白8抗体
合格 ZNF788 锌指蛋白788抗体
合格 ZNF79 锌指蛋白79抗体
合格 ZNF790 锌指蛋白790抗体
合格 ZNF785 锌指蛋白785抗体
合格 ZNF780A 锌指蛋白780A抗体
合格 Hemoglobin subunit delta 血红蛋白δ抗体
合格 phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) 磷酸化丝裂原活化蛋白激酶1/2抗体
合格 ZNF783 锌指蛋白783抗体
合格 ZNF773 锌指蛋白773抗体
合格 ZNF774 锌指蛋白774抗体
合格 ZNF776 锌指蛋白776抗体
合格 ZNF770 锌指蛋白770抗体
合格 ZFP771 锌指蛋白771抗体
合格 ZNF76 锌指蛋白76抗体
合格 ZNF763 锌指蛋白763抗体
合格 合格 HIATL1 HIATL1蛋白抗体
合格 phospho-P53(Ser392) 磷酸化肿瘤抑制基因P53抗体
合格 ZNF687 锌指蛋白687抗体
合格 ZNF676 锌指蛋白676抗体
合格 ZNF680 锌指蛋白680抗体
合格 ZNF683 锌指蛋白683抗体
合格 ZNF671 锌指蛋白671抗体
合格 ZNF673 锌指蛋白673抗体
合格 ZNF674 锌指蛋白674抗体
合格 HSD17B14 17β羟类固醇脱氢酶14/17β-HSD14抗体
合格 HSF4 热休克因子蛋白4抗体
合格 合格 HSFY1 热休克转录因子HSFY1抗体
合格 TRAF5 肿瘤坏死因子受体相关因子5抗体
合格 合格 TRAM1 相关易位膜蛋白1抗体
合格 TRANK1 TRANK1蛋白抗体
合格 Translin TSN蛋白抗体
合格 phospho-STAT1 (Tyr701) 磷酸化信号转导与转录激活因子1抗体