Phospho-Connexin 43 (Ser368)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3098R
英文名称Phospho-Connexin 43 (Ser368)
中文名称磷酸化Connexin 43蛋白抗体
别 名Connexin 43(phospho-Ser368); Connexin 43(phospho Ser368); Connexin 43(phospho S368); Connexin 43; Connexin43v Cx 43v CX43; DFNB38; Gap junction 43 kDa heart protein;Connexin-43; Gap junction alpha 1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; GJA1; GJA-1; GJAL; HGNC:4282; HGNC:8112; Oculodentodigital dysplasia; ODD; ODOD; SDTY3; Syndactyly type III; CXA1_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域心血管 细胞生物 神经生物学 信号转导 细胞粘附分子
抗体来源Rabbit
克隆类型Polyclonal
Phospho-Connexin 43 (Ser368)抗体交叉反应 Human, Mouse, Rat, Dog, Cow, Monkey,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Connexin 43 around the phosphorylation site of Ser368 [RA(p-S)SR]:RA(p-S)SR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Connexin 43 (Ser368)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq].
Function:
Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract.
Subunit:
A connexon is composed of a hexamer of connexins. Interacts (via C-terminus) with TJP1. Interacts (via C-terminus) with SRC (via SH3 domain). Interacts with UBQLN4. Interacts with SGSM3. Interacts with KIAA1432/CIP150. Interacts with CNST and CSNK1D.
Subcellular Location:
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Tissue Specificity:
Expressed in the heart and fetal cochlea.
Post-translational modifications:
Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity. Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly.
DISEASE:
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Similarity:
Belongs to the connexin family. Alpha-type (group II) subfamily.
Gene ID:
2697
Phospho-Connexin 43 (Ser368)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Connexin 43 (Ser368)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 RUSC2 RUSC2抗体
合格 VHL 脑视网膜血管瘤G7蛋白抗体（逢希伯-林道氏?。?br />
合格 合格 UBXN2B UBXN2B蛋白抗体
合格 WDR68 WDR68蛋白抗体
合格 C17orf87 第十七号染色体开放阅读框87抗体
合格 KAT5 / Tip60 TIP60抗体
合格 LAMTOR1 LAMTOR1蛋白抗体
合格 MS4A6A 四度跨膜蛋白3抗体
合格 PAG1 跨膜接头蛋白PAG抗体
合格 合格 PARD6G PARD6G蛋白抗体
合格 MAP2 微管相关蛋白2抗体
合格 SH3BGRL2 SH3BGRL2蛋白抗体
合格 LAD1 LAD1蛋白抗体
合格 合格 ATM ****扩张性共济失调症突变蛋白抗体
合格 PR3 白细胞蛋白酶3抗体
合格 CDH12 脑钙粘蛋白12抗体
合格 SNAIL Snail蛋白抗体
合格 PCDHB12 钙粘蛋白β12抗体
合格 Smoothelin 平滑肌蛋白抗体
合格 Vinexin 细胞间粘附蛋白Vinexin抗体
合格 CD99L2 CD99样蛋白2抗体
合格 Claudin 23 紧密连接蛋白23抗体
合格 Claudin 22 紧密连接蛋白22抗体
合格 Claudin 20 紧密连接蛋白20抗体
合格 Claudin 19 紧密连接蛋白19抗体
合格 Claudin 16 紧密连接蛋白16抗体
合格 C21orf66 21号染色体开放阅读框66抗体
合格 CDC42EP3 CDC42效应蛋白3抗体
合格 Ghrelin 28 脑肠肽抗体
合格 NUDCD3 NUDCD3蛋白抗体
合格 PABPC4L 聚腺苷酸养结合蛋白4抗体
合格 RCCD1 RCCD1蛋白抗体
合格 phospho-14-3-3 beta + zeta (Ser186 / Ser184) 磷酸化14-3-3 β/ζ抗体
合格 phospho-14-3-3 Tau (Ser232) 磷酸化14-3-3 τ抗体
合格 Phospho-5-Lipoxygenase(Ser524) 磷酸化5-脂氧合酶抗体
合格 C19orf25 19号染色体开放阅读框25抗体