SC35蛋白抗体
规格：1mg/1ml
英文名： SC35
别名： 35 kDa; arginine/serine-rich 2; PR264; Protein PR264; SC 35; SC-35; SC35; Serine/arginine-rich splicing factor 2; SFRS 2; SFRS2; SFRS2A; Splicing component 35 kDa; Splicing component; Splicing factor;
分子量： 25kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human SC35
交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Zebrafish, Guinea Pig,
细胞定位：细胞核
SC35蛋白抗体产品介绍：background: The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010] Function: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'SC35蛋白抗体- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. Subcellular Location: Nucleus. Post-translational modifications: Extensively phosphorylated on serine residues in the RS domain. Similarity: Belongs to the splicing factor SR family. Contains 1 RRM (RNA recognition motif) domain. Database links: Entrez Gene: SC35蛋白抗体508312 Cow Entrez Gene: 6427 Human Entrez Gene: 20382 Mouse Entrez Gene: 494445 Rat Omim: 600813 Human SwissProt: P30352 Chicken SwissProt: Q3MHR5 Cow SwissProt: Q01130 Human SwissProt: Q62093 Mouse SwissProt: Q6PDU1 Rat Unigene: 584801 Human Unigene: 21841 Mouse Unigene: 429312 Mouse Unigene: 136644 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
SC35蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid