TRIM72蛋白抗体
规格：1mg/1ml
英文名： TRIM72
别名： LOC493829; Mg53; Mitsugumin-53; TRI72_HUMAN; trim72; Tripartite motif containing 72; Tripartite motif containing protein 72; Tripartite motif-containing protein 72.
分子量： 53kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human TRIM72
交叉反应：Human, Mouse, Rat,
细胞定位：细胞膜
TRIM72蛋白抗体产品介绍：background: The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM72 (tripartite motif containing 72), also known as MG53, is a 477 amino acid cytoplasmic vesicle membrane protein that belongs to the TRIM/RBCC family. Existing as a homooligomer, TRIM72 contains one B box-type zinc finger, one B30.2/SPRY domain and a RING-type zinc finger. TRIM72 is considered a muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. TRIM72 is required for transport of dysferlin to sites of cell injury during repair patch formation. TRIM72 also regulates membrane budding and exocytosis and may be involved in the regulation of the mobility of KV2.1-containing endocytic vesicles. TRIM72 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p11.2. Function: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage,TRIM72蛋白抗体 entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. Subcellular Location: Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction TRIM72蛋白抗体with phosphatidylserine. Post-translational modifications: Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization. Similarity: Belongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. Database links: Entrez Gene: 467960 Chimpanzee Entrez Gene: 528804 Cow Entrez Gene: 489927 Dog Entrez Gene: 493829 Human Entrez Gene: 434246 Mouse Entrez Gene: 100008639 Rabbit Entrez Gene: 365377 Rat SwissProt: Q6ZMU5 Human SwissProt: Q1XH17 Mouse SwissProt: Q1XH18 Rabbit SwissProt: A0JPQ4 Rat Unigene: 661859 Human Unigene: 389924 Mouse Unigene: 23565 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
TRIM72蛋白抗体研究领域：细胞生物 **学 信号转导
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid